Adams S M, Helps N R, Sharp M G, Brammar W J, Walker R A, Varley J M
ICI/University Joint Laboratory, University of Leicester, UK.
Hum Mol Genet. 1992 May;1(2):91-6. doi: 10.1093/hmg/1.2.91.
We report the identification of a novel cDNA representing an mRNA showing significantly higher levels of expression in benign breast lesions than in carcinomas. This cDNA was identified by differential screening of a cDNA library generated from a breast carcinoma, and shows consistently higher expression in fibroadenomas than in carcinomas. The expression in both benign and malignant tissues is highest in epithelial cells as determined by in situ hybridization to tissue sections. The nucleotide sequence of the full-length cDNA has been determined, and the deduced protein is highly basic with no signal or transmembrane sequence, but two potential nuclear localization signals. Neither the DNA nor the protein sequence show any significant homology to sequences in current databases. The cDNA hybridizes to multiple sequences within both human and other mammalian genomes, but to single genomic sequences in Drosophila, Physarum and Schizosaccharomyces pombe. This cDNA therefore represents a highly conserved gene sequence. We have identified only one major transcript in human cells, and it seems likely that there are several pseudogenes within the human genome.
我们报告了一个新cDNA的鉴定结果，该cDNA所代表的mRNA在良性乳腺病变中的表达水平显著高于癌组织。这个cDNA是通过对乳腺癌来源的cDNA文库进行差异筛选鉴定出来的，在纤维腺瘤中的表达始终高于癌组织。通过对组织切片进行原位杂交确定，该基因在良性和恶性组织的上皮细胞中表达最高。已测定了全长cDNA的核苷酸序列，推导的蛋白质具有高度碱性，没有信号序列或跨膜序列，但有两个潜在的核定位信号。DNA序列和蛋白质序列与当前数据库中的序列均无明显同源性。该cDNA与人类及其他哺乳动物基因组中的多个序列杂交，但仅与果蝇、绒泡菌和粟酒裂殖酵母中的单个基因组序列杂交。因此，这个cDNA代表了一个高度保守的基因序列。我们在人类细胞中仅鉴定出一个主要转录本，人类基因组中似乎存在几个假基因。
