Vil'chinkas R L, Elanskaia I V
Mol Gen Mikrobiol Virusol. 1992 Nov-Dec(11-12):8-10.
The XhoI-SalGI fragment of the plasmid pCI DNA was inserted into the SalGI site of the cyanobacterium Anacystis nidulans R2 integrative vector plasmid pIAH4. The fragment incorporates the endoglucanase gene of Clostridium thermocellum cloned earlier within the 6.7 kb DNA sequence. The recombinant plasmid DNA was transformed into Anacystis nidulans R2 cells. The cloned endoglucanase gene was shown to express in the cyanobacterium cells. The enzyme synthesized is accumulated within the cytoplasm of Anacystis nidulans cells and is not secreted into the periplasm.
将质粒pCI DNA的XhoI-SalGI片段插入蓝藻集胞藻6803 R2整合载体质粒pIAH4的SalGI位点。该片段包含先前克隆在6.7 kb DNA序列内的嗜热栖热放线菌内切葡聚糖酶基因。将重组质粒DNA转化到集胞藻6803 R2细胞中。结果表明，克隆得到的内切葡聚糖酶基因在蓝藻细胞中表达。合成的酶在集胞藻细胞的细胞质中积累，而不分泌到周质中。
