[能够整合到蓝藻集胞藻6803染色体中的重组质粒]
[Recombinant plasmids capable of integration into the chromosome of the cyanobacterium Anacystis nidulans R2].
作者信息
Elanskaia I V, Morzunova I B, Shestakov S V
出版信息
Mol Gen Mikrobiol Virusol. 1985 Nov(11):20-4.
PMID:3025685
Abstract
Recombinant plasmids, series pIAB and pIAH, have been constructed by insertion of BamHI or HindIII chromosomal fragments from Anacystis nidulans R2 into the tet gene of plasmid pACYC184. Plasmids pIAB and pIAH are stably maintained in Escherichia coli cells and transfer the CmR marker in transformation of Anacystis nidulans. Blot hybridization technique has shown the formation of CmR clones in transformation to result from integration of plasmid pACYC184 with the chromosome of cyanobacterium.
摘要
通过将来自集胞藻6803 R2的BamHI或HindIII染色体片段插入质粒pACYC184的tet基因中，构建了重组质粒pIAB和pIAH系列。质粒pIAB和pIAH在大肠杆菌细胞中稳定维持，并在集胞藻6803转化过程中转移CmR标记。印迹杂交技术表明，转化中CmR克隆的形成是由于质粒pACYC184与蓝细菌染色体整合所致。
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