McLachlan A, Shaw K J, Hocking A D, Pitt J I, Nguyen T H
CSIRO Division of Food Processing, North Ryde, NSW, Australia.
Food Addit Contam. 1992 Nov-Dec;9(6):631-8. doi: 10.1080/02652039209374118.
Australian isolates of Fusarium species were grown on potato dextrose agar. Trichothecenes produced by these species were extracted by ethyl acetate followed by methanol and a silica gel column was used to clean-up the extract. The extracted samples were derivatized by acetylation with trifluoroacetic anhydride and the derivatives analysed by gas chromatography/mass spectrometry (GC/MS). Multiple ion detection was used to trace ions characteristic of the trichothecenes expected to be present. Quantitation of those found was based on a known mass of pentabromophenol that was added as an internal standard. Eight species of Fusarium (nineteen strains) were surveyed, of which three species, F. acuminatum, F. equiseti and F. sporotrichioides, produced the trichothecenes scirpentriol, diacetoxyscirpenol, neosolaniol, HT-2 toxin, T-2 toxin, T-2 tetraol and deoxynivalenol. Wheat samples were inoculated with four different species of Fusarium, F. acuminatum, F. equiseti, F. graminearum and F. sporotrichioides, and in these samples diacetoxyscirpenol, neosolaniol, HT-2 toxin and T-2 toxin were found.
将澳大利亚分离出的镰刀菌属菌种在马铃薯葡萄糖琼脂培养基上培养。这些菌种产生的单端孢霉烯族毒素先用乙酸乙酯萃取，接着用甲醇萃取，然后使用硅胶柱对提取物进行净化处理。提取的样品用三氟乙酸酐乙酰化进行衍生化，衍生化产物通过气相色谱/质谱联用仪（GC/MS）进行分析。采用多离子检测来追踪预期存在的单端孢霉烯族毒素的特征离子。对所发现毒素的定量分析基于加入的已知质量的五溴苯酚作为内标物。对8种镰刀菌（19个菌株）进行了调查，其中尖孢镰刀菌、木贼镰刀菌和拟枝孢镰刀菌这3个菌种产生了蛇形菌素、二醋酸蛇形菌素、新茄病镰刀菌烯醇、HT-2毒素、T-2毒素、T-2四醇和脱氧雪腐镰刀菌烯醇。用尖孢镰刀菌、木贼镰刀菌、禾谷镰刀菌和拟枝孢镰刀菌这4种不同的镰刀菌对小麦样品进行接种，在这些样品中发现了二醋酸蛇形菌素、新茄病镰刀菌烯醇、HT-2毒素和T-2毒素。
