Parente A H, Marques E T, Azevedo W M, Diniz F B, Melo E H, Lima Filho J L
Departamento de Bioquimica, Laboratorio de Imunopatologia, Keizo Asami-LIKA, Brazil.
Appl Biochem Biotechnol. 1992 Dec;37(3):267-73. doi: 10.1007/BF02788878.
A biosensor for glucose utilizing kinetics of glucose oxidase (EC 1.1.3.4.) was developed. The enzyme was immobilized on polyaniline by covalent bonding, using glutaraldehyde as a bifunctional agent. The system showed a linear response up to 2.2 mM of glucose with a response time of 2.5-4.0 min. In addition, the immobilized enzyme had a higher activity between pH 6.5 and 7.5. The system retained 50% of its activity after 30 d of daily use. The optical absorption spectra of the polyaniline/glucose oxidase electrode after glucose had been added to the buffer solution showed that the absorption band around 800 nm had changed considerably when glucose was allowed to react with the electrode. This optical variation makes polyaniline a very promising polymer for use as a support in optical sensor for clinical application.
开发了一种利用葡萄糖氧化酶（EC 1.1.3.4.）动力学的葡萄糖生物传感器。使用戊二醛作为双功能试剂，通过共价键将该酶固定在聚苯胺上。该系统在高达2.2 mM的葡萄糖浓度下呈现线性响应，响应时间为2.5 - 4.0分钟。此外，固定化酶在pH 6.5至7.5之间具有更高的活性。该系统在每日使用30天后仍保留其50%的活性。向缓冲溶液中添加葡萄糖后，聚苯胺/葡萄糖氧化酶电极的光吸收光谱表明，当葡萄糖与电极反应时，800 nm左右的吸收带发生了显著变化。这种光学变化使聚苯胺成为一种非常有前途的聚合物，可作为临床应用光学传感器的支撑材料。
