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[工作场所空气中乙酰辛浓度的光度法和色谱法测定]

[Photometric and chromatographic methods of measuring the concentration of acelysin in the air of a work area].

作者信息

Ivaniuk E G, Kolievskaia Iu A, Pisareva N E, Mikhaĭlik S V, Perets I D

出版信息

Gig Tr Prof Zabol. 1992(11-12):35-7.

PMID:1303395
Abstract

Two methods were applied to evaluation of acelysine level. Spectrophotometric one was based on reaction with p-nitrophenyldiasonium and evaluation of the product optic density at 510 nm. The second was chromatography in thin layer, where a mixture of n-butanol, formic acid and water was used as an eluent and 0.2% ninhydrin solution in butanol and 3 ml of ice acetic acid (60-70 degrees C) served as a developer. Both methods are supposed to evaluate acelysine level in the air of working area, when a half of MAC and admixtures are present.

摘要

两种方法用于评估乙酰赖氨酸水平。分光光度法基于与对硝基苯重氮盐的反应,并在510nm处评估产物的光密度。第二种方法是薄层色谱法,其中使用正丁醇、甲酸和水的混合物作为洗脱剂,0.2%茚三酮丁醇溶液和3ml冰醋酸(60-70℃)用作显色剂。当存在一半的职业接触限值(MAC)和混合物时,两种方法都应评估工作区域空气中的乙酰赖氨酸水平。

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