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针对绒泡菌肌球蛋白的抗体。I. 制备及功能效应

Antibody to Physarum myosin. I. Preparation and functional effects.

作者信息

Nachmias V T, Kessler D

出版信息

Immunology. 1976 Mar;30(3):419-24.

PMID:130343
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1445155/
Abstract

Preparation of antibody to Physarum myosin is described, and evidence is presented that the antibody is specific for this molecule. A diffusion coefficient of 1 X 10(-7) cm2/s is estimated. The antibody interfered with myosin enzyme activity and with superprecipitation of actomyosin. It did not cross-react with rabbit striated muscle myosin.

摘要

描述了针对绒泡菌肌球蛋白抗体的制备,并提供证据表明该抗体对该分子具有特异性。估计扩散系数为1×10⁻⁷ cm²/s。该抗体干扰了肌球蛋白酶活性和肌动球蛋白的超沉淀。它与兔横纹肌肌球蛋白没有交叉反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e66/1445155/8927908b4132/immunology00302-0108-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e66/1445155/5ddf1554152a/immunology00302-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e66/1445155/c13a1715f3e6/immunology00302-0108-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e66/1445155/8927908b4132/immunology00302-0108-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e66/1445155/5ddf1554152a/immunology00302-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e66/1445155/c13a1715f3e6/immunology00302-0108-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e66/1445155/8927908b4132/immunology00302-0108-c.jpg

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引用本文的文献

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本文引用的文献

1
Immunogenetic analysis of proteins of Paramecium. I. Comparison of specificities controlled by alleles and by different loci.草履虫蛋白质的免疫遗传学分析。I. 等位基因和不同基因座所控制特异性的比较。
Genetics. 1962 Feb;47(2):223-39. doi: 10.1093/genetics/47.2.223.
2
The pure culture of Physarum polycephalum on a partially defined soluble medium.多头绒泡菌在部分限定的可溶性培养基上的纯培养物。
J Gen Microbiol. 1961 May;25:47-59. doi: 10.1099/00221287-25-1-47.
3
A quantitative study of a technique of double diffusion in agar.琼脂中双扩散技术的定量研究。
J Immunol. 1956 Jul;77(1):52-60.
4
The myosin filament. II. Interaction between myosin and actin filaments observed using antibody staining in fluorescent and electron microscopy.肌球蛋白丝。II. 使用荧光和电子显微镜下的抗体染色观察到的肌球蛋白与肌动蛋白丝之间的相互作用。
J Mol Biol. 1967 Jul 28;27(2):227-36. doi: 10.1016/0022-2836(67)90017-4.
5
Properties of Physarum myosin purified by a potassium iodide procedure.通过碘化钾法纯化的绒泡菌肌球蛋白的特性。
J Cell Biol. 1974 Jul;62(1):54-65. doi: 10.1083/jcb.62.1.54.
6
Filament formation by purified Physarum myosin.纯化的绒泡菌肌球蛋白形成细丝。
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2011-4. doi: 10.1073/pnas.69.8.2011.
7
Further purification and characterization of slime mold myosin and slime mold actin.黏菌肌球蛋白和黏菌肌动蛋白的进一步纯化及特性研究。
Biochemistry. 1969 Dec;8(12):4976-88. doi: 10.1021/bi00840a047.
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J Cell Biol. 1966 Mar;28(3):505-25. doi: 10.1083/jcb.28.3.505.