Lafosse M, Kéravis G, Durand M H
J Chromatogr. 1976 Apr 7;118(3):283-94. doi: 10.1016/s0021-9673(00)82165-2.
High-pressure liquid chromatography (HPLC) should be used more frequently in biomedical research because heavy molecules can be analysed at ambient temperature without derivatisation to volatile compounds. In this paper the separation of four free 17-ketosteroids, their ester sulfates and glucuronides by reversed-phase HPLC on a Micropak CH column is described. In order to improve the separation several parameters have been studied. Retention data allow comparison of the hydrophilic behaviour of free and conjugated steroids.
高效液相色谱法（HPLC）在生物医学研究中应更频繁地使用，因为大分子可以在室温下进行分析，无需衍生为挥发性化合物。本文描述了在Micropak CH柱上通过反相高效液相色谱法分离四种游离17-酮类固醇、它们的硫酸酯和葡萄糖醛酸苷。为了改善分离效果，研究了几个参数。保留数据有助于比较游离和结合类固醇的亲水行为。
