Pardo A, Ramirez R, Ramos C, Montaãno M, Selman M
Facultad de Ciencias, Universidad Nacional Autónoma de México, DF.
Connect Tissue Res. 1992;28(4):307-15. doi: 10.3109/03008209209016822.
The levels of collagenase inhibitor, both free and bound to metalloproteinases, were evaluated at 7 days [deposit phase (DP)] and 14 days [resorptive phase (RP)] of evolution of the subcutaneous carrageenin-induced granuloma in the guinea pig. The level of free collagenase inhibitor was considerably higher in the supernatant of DP granulomas (7.95 +/- 1.53 U/mg protein) as compared to that of RP granulomas (2.53 +/- 0.41 U/mg protein). When the samples were heated at acid pH to release the inhibitor from metalloproteinase-inhibitor complexes, free inhibitor was recovered in both phases. However, the units of recovered collagenase inhibitor were several fold higher in all RP granulomas in comparison with DP granulomas (6.88 +/- 2.46 vs 1.5 +/- 0.53). Therefore, DP and RP tissues exhibited similar total amount of tissue inhibitor. By HPLC, collagenase inhibitor activity was localized in a fraction consistent with the size of TIMP. These results suggest a different balance of collagenase and collagenase inhibitor during the evolution of the granuloma; an excess of inhibitor over metalloproteinases appears to predominate during the phase of collagen accumulation contrasting with an inverse situation when the granuloma is healing.
在豚鼠皮下角叉菜胶诱导的肉芽肿演变的第7天[沉积期（DP）]和第14天[吸收期（RP）]，评估了游离的和与金属蛋白酶结合的胶原酶抑制剂水平。与RP肉芽肿（2.53±0.41 U/mg蛋白质）相比，DP肉芽肿上清液中游离胶原酶抑制剂水平显著更高（7.95±1.53 U/mg蛋白质）。当样品在酸性pH下加热以从金属蛋白酶-抑制剂复合物中释放抑制剂时，两个阶段均回收了游离抑制剂。然而，所有RP肉芽肿中回收的胶原酶抑制剂单位比DP肉芽肿高几倍（6.88±2.46对1.5±0.53）。因此，DP和RP组织表现出相似的组织抑制剂总量。通过高效液相色谱法，胶原酶抑制剂活性定位于与金属蛋白酶组织抑制因子（TIMP）大小一致的组分中。这些结果表明在肉芽肿演变过程中胶原酶和胶原酶抑制剂的平衡不同；在胶原积累阶段，抑制剂相对于金属蛋白酶过量似乎占主导，这与肉芽肿愈合时的相反情况形成对比。
