胶原酶 - 金属蛋白酶组织抑制剂 -1（TIMP -1）复合物的解离——其在粗培养液和体液中独立测定TIMP -1和胶原酶活性的应用
Dissociation of collagenase-tissue inhibitor of metalloproteinases-1 (TIMP-1) complex--its application for the independent measurements of TIMP-1 and collagenase activity in crude culture media and body fluids.
作者信息
Yamashita K, Zhang J, Zou L, Hayakawa H, Noguchi T, Kondo I, Narita O, Fujimoto N, Iwata K, Hayakawa T
机构信息
Department of Biochemistry, School of Dentistry, Aichi-Gakuin University, Nagoya, Japan.
出版信息
Matrix. 1992 Dec;12(6):481-7. doi: 10.1016/s0934-8832(11)80093-4.
Collagenase-tissue inhibitor of metalloproteinases-1 (TIMP-1) complex was prepared from activated collagenase and TIMP-1 purified from culture media of human skin fibroblasts. After having been confirmed to be a complex by zinc chelate chromatography, the complex was demonstrated to dissociate by passage through an anti-TIMP-1 monoclonal antibody-affinity column. On the basis of above evidence, a simple strategy was set up for the independent measurements of TIMP-1 concentration, and both active and total collagenase activities in crude culture media and body fluids.
从活化的胶原酶和从人皮肤成纤维细胞培养基中纯化得到的金属蛋白酶组织抑制剂-1（TIMP-1）制备了胶原酶-TIMP-1复合物。通过锌螯合色谱法确认其为复合物后，该复合物经抗TIMP-1单克隆抗体亲和柱处理后被证明会解离。基于上述证据，建立了一种简单的策略，用于独立测量粗培养介质和体液中TIMP-1的浓度以及活性和总胶原酶活性。
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