氧化葡萄糖酸杆菌CCRC 10383生产头孢氨苄

Production of cephalexin by Gluconobacter oxydans CCRC 10383.

作者信息

Liu Y T, Lee S H

机构信息

Department of Microbiology and Immunology, National Defense Medical Center, Taipei, Taiwan, Republic of China.

出版信息

Proc Natl Sci Counc Repub China B. 1992 Oct;16(4):184-7.

PMID:1305772

Abstract

Intact cells of Gluconobacter oxydans CCRC 10383 produced cephalexin from 7-amino-3-deacetoxy cephalosporanic acid (7-ADCA) and D-alpha-phenylglycine methylester HC1 (PGM). Factors affecting the production of cephalexin by G. oxydans CCRC 10383 were studied. The optimum pH and temperature for the synthetic reaction of cephalexin were 6.0 and 42 degrees C, respectively. A higher concentration of PGM than 7-ADCA was required to obtain a good yield of cephalexin.

摘要

氧化葡萄糖杆菌CCRC 10383的完整细胞可由7-氨基-3-去乙酰氧基头孢烷酸（7-ADCA）和D-α-苯甘氨酸甲酯盐酸盐（PGM）生产头孢氨苄。研究了影响氧化葡萄糖杆菌CCRC 10383生产头孢氨苄的因素。头孢氨苄合成反应的最适pH和温度分别为6.0和42℃。为了获得较高的头孢氨苄产量，需要PGM的浓度高于7-ADCA。