Takeuchi Y, Fujiwara K, Ishimura K, Aoki S, Okano S, Yamazoe I, Yoshioka H, Sawada T
Department of Pediatrics, Kyoto Prefectural University of Medicine, Japan.
Dev Neurosci. 1992;14(5-6):394-402. doi: 10.1159/000111688.
The effects of neonatal asphyxia on the serotonin neuron system were examined using the immunoperoxidase method. Male mice, 2 days of age, were exposed to total asphyxia (100% CO2) for 30 min. Mice that spontaneously survived were perfused transcardially with a fixative at 15, 30 and 60 days of age. Quantitative immunohistochemical analysis at 60 days of age demonstrated a significant decrease in the numbers of serotonin-immunoreactive cell bodies in the nucleus raphe dorsalis, the nucleus raphe pontis, the subpyramidal region, the total raphe system and the whole brain, while no significant reduction in the number of serotonin-immunoreactive cell bodies was observed in the caudal raphe system. Presumably degenerative changes in serotonin-immunoreactive fibers were observed in various parts of the brain of mice subjected to total asphyxia at 15 days of age, and the numbers of degenerated fibers decreased in almost all parts of the brain, the exception being the caudal portion of the brainstem, at 30 and 60 days of age. These results suggested that neonatal asphyxia induced permanent changes in the serotonin neuron system, with regional differences.
采用免疫过氧化物酶法研究了新生儿窒息对5-羟色胺神经元系统的影响。选用2日龄雄性小鼠，使其暴露于完全窒息（100%二氧化碳）环境中30分钟。对自发存活的小鼠在15日龄、30日龄和60日龄时经心脏灌注固定液。60日龄时的定量免疫组织化学分析表明，中缝背核、脑桥中缝核、锥体下区域、整个中缝系统和全脑中5-羟色胺免疫反应性细胞体数量显著减少，而尾侧中缝系统中5-羟色胺免疫反应性细胞体数量未见明显减少。在15日龄时经历完全窒息的小鼠大脑各部位观察到5-羟色胺免疫反应性纤维可能的退行性变化，到30日龄和60日龄时，几乎大脑所有部位的退变纤维数量均减少，但脑干尾侧部分除外。这些结果表明，新生儿窒息可导致5-羟色胺神经元系统发生永久性变化，且存在区域差异。
