Zahm J M, Pierrot D, Chevillard M, Puchelle E
INSERM U 314, Université de Reims, France.
Biorheology. 1992 Sep-Dec;29(5-6):459-65. doi: 10.3233/bir-1992-295-606.
Epithelial wound repair represents an important process by which the epithelial barrier integrity recovers after wounding. To evaluate and quantify the dynamics of surface airway cell movement during the wound repair process, we developed an in vitro wounding model of human respiratory cells in culture and we analyzed the wound repair by using videomicroscopic and image analysis techniques. We observed that wound closure occurred within 6 hours, due to the spreading and migration of the cells surrounding the wounded surface. The migration rate of the cells at the leading edge of the wound surface increased progressively up to 26 microns/h during the repair process which was characterized by a uniform centripetal direction of cell movement. The distance travelled by these cells was 2.5 fold longer than the distance travelled by ciliated cells which were located far from the wound area. These results suggest that cell migration after wounding is an important process by which the respiratory epithelial barrier integrity is maintained.
上皮伤口修复是上皮屏障完整性在受伤后得以恢复的一个重要过程。为了评估和量化伤口修复过程中气道表面细胞运动的动态变化，我们建立了一种体外培养的人呼吸道细胞伤口模型，并使用视频显微镜和图像分析技术分析伤口修复情况。我们观察到，由于受伤表面周围细胞的铺展和迁移，伤口在6小时内闭合。在修复过程中，伤口表面前沿细胞的迁移速率逐渐增加，最高可达26微米/小时，其特征是细胞运动方向呈均匀的向心方向。这些细胞移动的距离比远离伤口区域的纤毛细胞移动的距离长2.5倍。这些结果表明，受伤后的细胞迁移是维持呼吸道上皮屏障完整性的一个重要过程。
