Tsuruta T, Masuko S, Watanabe H
Department of Surgery, Saga Medical School, Japan.
Tohoku J Exp Med. 1992 Dec;168(4):549-60. doi: 10.1620/tjem.168.549.
Immunohistochemical staining of arteries supplying the dog forepaw showed a dense distribution of nerve fibers which were immunoreactive to tyrosine hydroxylase (TH), neuropeptide Y (NPY), vasoactive intestinal peptide (VIP), substance P (SP), and calcitonin gene-related peptide (CGRP) around the vascular walls. The density of each immunoreactive fiber tended to increase in the peripheral branch of the vascular tree. Retrograde axonal tracing with Fast Blue from the artery revealed that these immunoreactive fibers originated from NPY-containing catecholaminergic as well as VIP/SP/CGRP-containing non-catecholaminergic neurons in the stellate ganglion and SP/CGRP-containing neurons in the dorsal root ganglia of segments C7 to Th1. After stellate ganglionectomy, TH-, NPY-, and, VIP-immunoreactive fibers disappeared completely from the arterial walls while approximately 40% of SP- and CGRP-immunoreactive fibers remained. The present results indicate that the artery of the dog forepaw receive triple innervation of adrenergic sympathetic, non-adrenergic sympathetic, and sensory fibers, and suggest that about 40% of SP- and CGRP-immunoreactive fibers are of sensory origin.
对供应犬前爪的动脉进行免疫组织化学染色显示，血管壁周围存在密集分布的神经纤维，这些神经纤维对酪氨酸羟化酶（TH）、神经肽Y（NPY）、血管活性肠肽（VIP）、P物质（SP）和降钙素基因相关肽（CGRP）呈免疫反应性。在血管树的外周分支中，每种免疫反应性纤维的密度都有增加的趋势。用固蓝对动脉进行逆行轴突追踪显示，这些免疫反应性纤维起源于星状神经节中含NPY的儿茶酚胺能神经元以及含VIP/SP/CGRP的非儿茶酚胺能神经元，以及C7至Th1节段背根神经节中含SP/CGRP的神经元。星状神经节切除术后，动脉壁上的TH、NPY和VIP免疫反应性纤维完全消失，而约40%的SP和CGRP免疫反应性纤维仍然存在。目前的结果表明，犬前爪的动脉接受肾上腺素能交感神经、非肾上腺素能交感神经和感觉纤维的三重支配，并提示约40%的SP和CGRP免疫反应性纤维起源于感觉神经。
