Hino N, Masuko S, Katsuki T
Department of Oral and Maxillofacial Surgery, Saga Medical School, Japan.
Arch Histol Cytol. 1993 Dec;56(5):505-16. doi: 10.1679/aohc.56.505.
The distribution, pathways and origins of peptide-containing nerve fibers in the anterior two thirds of the dog tongue were investigated using immunohistochemistry combined with retrograde axonal tracing and denervation experiments. Within the epithelium of the fungiform papillae, varicose nerve fibers immunoreactive to substance P (SP) and calcitonin gene-related peptide (CGRP) were present. These disappeared completely after severance of the lingual nerve (LN) alone. Dense CGRP-immunoreactive varicose fibers surrounded cell bodies in the intralingual ganglia (ILG), which consisted of neurons immunoreactive to vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY) and SP. These CGRP-immunoreactive fibers disappeared following severance of the chorda tympani (CT) alone. SP-, CGRP-, VIP-, NPY- and tyrosine hydroxylase (TH)-immunoreactive nerve fibers were distributed around the walls of blood vessels, especially arteriovenous anastomoses (AVAs). None of these immunoreactive fibers completely disappeared after severance of the LN or CT alone, but SP- and CGRP-immunoreactive fibers disappeared following severance of both the LN and CT. TH-immunoreactive fibers disappeared after ganglionectomy of the superior cervical ganglion (SCG) or severance of the hypoglossal nerve (HGN). VIP- and NPY-immunoreactive fibers invariably remained after various denervation experiments. In tracing experiments, CGRP-immunoreactive as well as SP and CGRP-immunoreactive cells in the trigeminal ganglion were labelled from the LN, and those in the geniculate ganglion and jugular ganglion were labelled from the CT. A large number of neurons in the SCG were labelled from the HGN, with some of these being SP and CGRP-immunoreactive. These results demonstrate that SP- and CGRP-immunoreactive fibers from the trigeminal ganglion are distributed to the lingual epithelium; vascular walls receive SP- and CGRP-immunoreactive sensory fibers from the LN as well as CT, some SP and CGRP-immunoreactive fibers from the SCG in addition to catecholaminergic sympathetic fibers, and VIP- and NPY-immunoreactive parasympathetic fibers from the ILG. The ILG is also considered to be innervated by CGRP-immunoreactive sensory fibers from the CT.
采用免疫组织化学结合逆行轴突追踪和去神经支配实验，研究了犬舌前三分之二含肽神经纤维的分布、走行和起源。在菌状乳头的上皮内，存在对P物质（SP）和降钙素基因相关肽（CGRP）免疫反应的曲张神经纤维。单独切断舌神经（LN）后，这些纤维完全消失。密集的CGRP免疫反应性曲张纤维围绕着舌内神经节（ILG）中的细胞体，该神经节由对血管活性肠肽（VIP）、神经肽Y（NPY）和SP免疫反应的神经元组成。单独切断鼓索神经（CT）后，这些CGRP免疫反应性纤维消失。SP、CGRP、VIP、NPY和酪氨酸羟化酶（TH）免疫反应性神经纤维分布在血管壁周围，尤其是动静脉吻合处（AVA）。单独切断LN或CT后，这些免疫反应性纤维均未完全消失，但同时切断LN和CT后，SP和CGRP免疫反应性纤维消失。切断颈上神经节（SCG）或舌下神经（HGN）后，TH免疫反应性纤维消失。在各种去神经支配实验后，VIP和NPY免疫反应性纤维始终存在。在追踪实验中，三叉神经节中的CGRP免疫反应性以及SP和CGRP免疫反应性细胞由LN标记，膝状神经节和颈静脉神经节中的细胞由CT标记。大量SCG中的神经元由HGN标记，其中一些为SP和CGRP免疫反应性。这些结果表明，来自三叉神经节的SP和CGRP免疫反应性纤维分布至舌上皮；血管壁接受来自LN和CT的SP和CGRP免疫反应性感感觉纤维、除儿茶酚胺能交感纤维外来自SCG的一些SP和CGRP免疫反应性纤维以及来自ILG的VIP和NPY免疫反应性副交感纤维。ILG也被认为接受来自CT的CGRP免疫反应性感感觉纤维的支配。
