Ono M, Matsuda T, Abe K, Itoh C
Department of Clinical Pathology, Iwate Medical University, Morioka.
Rinsho Byori. 1992 Oct;40(10):1085-8.
Cytokines production by OK-432-stimulated peripheral blood mononuclear cells (PBMC) were measured to investigate the in vitro function of macrophages (M phi) and lymphocytes. PBMC (1 x 10(6) cells/ml) were cultured with OK-432 (0.05 KE/ml) for 72 hr at 37 degrees C under 5% CO2, then interleukin 1 beta (IL-1 beta), interleukin 2 (IL-2) and soluble IL-2 receptor (sIL-2R) levels in the culture supernatants were measured by ELISA. While there was no significant differences of IL-1 beta production between patients with chronic active hepatitis type B (CAH-B) and controls, sIL-2R production (335 +/- 219 U/ml, mean +/- SD) was significantly decreased (p < 0.001) in patients with CAH-B. On the other hand, in pregnant women, production of both IL-1 beta (6.3 +/- 3.9 ng/ml, p < 0.01) and sIL-2R (300 +/- 169 U/ml, p < 0.001) were significantly lower than those in controls (13.5 +/- 3.8 ng/ml, 969 +/- 154 U/ml). These results suggest that the expression of IL-2R alpha on lymphocytes membrane is suppressed in patients with CAH-B, and that decreased M phi function is present in pregnant women.
通过测量OK-432刺激的外周血单核细胞（PBMC）产生的细胞因子，来研究巨噬细胞（M phi）和淋巴细胞的体外功能。将PBMC（1×10⁶个细胞/ml）与OK-432（0.05 KE/ml）在37℃、5%二氧化碳条件下培养72小时，然后通过酶联免疫吸附测定法（ELISA）测量培养上清液中白细胞介素1β（IL-1β）、白细胞介素2（IL-2）和可溶性IL-2受体（sIL-2R）的水平。虽然慢性乙型活动性肝炎（CAH-B）患者和对照组之间IL-1β的产生没有显著差异，但CAH-B患者的sIL-2R产生（335±219 U/ml，平均值±标准差）显著降低（p<0.001）。另一方面，在孕妇中，IL-1β（6.3±3.9 ng/ml，p<0.01）和sIL-2R（300±169 U/ml，p<0.001）的产生均显著低于对照组（13.5±3.8 ng/ml，969±154 U/ml）。这些结果表明，CAH-B患者淋巴细胞膜上IL-2Rα的表达受到抑制，且孕妇存在M phi功能降低的情况。
