Kuroso K, Isogai N, Koike K, Fukutake K, Fujimaki M
Department of Clinical Pathology, Tokyo Medical College.
Rinsho Byori. 1992 Dec;40(12):1281-6.
We evaluated the clinical significance of two novel assays for the detection of crosslinked fibrin degradation products (XDP) in whole blood using the agglutination of the red blood cells (SimpliRED D dimer and SimpliRED D dimer-500, AGEN, Australia). XDP made serially by plasmin in vitro, were detected by the SimpliRED D dimer assay, but fibrinogen degradation products showed weak reactivity. Ten of the fifty four clinical samples collected with EDTA-2K, changed to positive on these assays after overnight incubation at 4 degrees C. Anemia and hemolytic samples had no effect on the assay results. The results obtained by the SimpliRED D dimer were negative for the normal subjects (n = 50) without exception. In our study, 81% and 95% of the patients, who showed abnormal levels of XDP in plasma and E fragments in serum respectively, were positive on the SimpliRED D dimer assay. The assay was as sensitive as the Rapidia-D dimer assay. In conclusion, the SimpliRED D dimer assay was clinically useful as a screening assay for the diagnosis of hypercoagulable and fibrinolytic states, since it could be performed simply and quickly.
我们使用红细胞凝集法（澳大利亚AGEN公司的SimpliRED D二聚体和SimpliRED D二聚体-500）评估了两种新型检测方法在全血中检测交联纤维蛋白降解产物（XDP）的临床意义。体外纤溶酶连续生成的XDP可通过SimpliRED D二聚体检测法检测到，但纤维蛋白原降解产物的反应性较弱。用乙二胺四乙酸二钾（EDTA-2K）采集的54份临床样本中有10份，在4℃过夜孵育后这些检测呈阳性。贫血和溶血样本对检测结果无影响。正常受试者（n = 50）的SimpliRED D二聚体检测结果无一例外均为阴性。在我们的研究中，血浆中XDP水平异常和血清中E片段异常的患者分别有81%和95%在SimpliRED D二聚体检测中呈阳性。该检测与Rapidia-D二聚体检测一样敏感。总之，SimpliRED D二聚体检测作为一种筛查检测方法，可简单快速地进行，在诊断高凝状态和纤溶状态方面具有临床实用性。
