Gregoraszczuk E L
Department of Animal Physiology, Jagiellonian University, Krakow, Poland.
Endocr Regul. 1992 Dec;26(4):189-94.
Four types of porcine corpora lutea (CL) were excised at 4 various stages of the luteal phase. One part of corpora lutea was homogenized and the concentration of progesterone (P4), estradiol (E2) and androgens (A) was assayed in adequate extracts. Second part of luteal cells obtained from each of four types of CL was cultured as separate monolayers and steroid secretion was measured after 24, 48 and 96 h of culture. The concentration of P4 in homogenates of CL type 2 and 3 was twice as high as that in type 1, while a decrease to minimum level in type 4 was observed. In cultures, maximum increase in P4 production was observed after 24 h, being the highest in type 1 (2800% of initial P4 concentration) and type 2 (320%) while in types 3 and 4 no increase was observed. The contents of androgens (A) in CL homogenates increased 3 times in type 2 and 3 and finally decreased in type 4 as compared with type 1. In cultures, maximum increase in A production was observed after 24 h, being the highest in type 1 (640% of A concentration prior to culture), while in types 2, 3 and 4 it was about 200%. The content of E2 in homogenates of CL types 1 and 2 was very low, but increased to about 200% in type 3 and to about 2500% in type 4.(ABSTRACT TRUNCATED AT 250 WORDS)
在黄体期的4个不同阶段切除四种类型的猪黄体。将一部分黄体匀浆，并在适当的提取物中测定孕酮（P4）、雌二醇（E2）和雄激素（A）的浓度。从四种类型的黄体中获得的第二部分黄体细胞作为单独的单层培养，培养24、48和96小时后测量类固醇分泌。2型和3型黄体匀浆中P4的浓度是1型的两倍，而4型则降至最低水平。在培养物中，24小时后观察到P4产量的最大增加，1型（初始P4浓度的2800%）和2型（320%）最高，而3型和4型没有增加。与1型相比，2型和3型黄体匀浆中雄激素（A）的含量增加了3倍，4型最终下降。在培养物中，24小时后观察到A产量的最大增加，1型（培养前A浓度的640%）最高，而2型、3型和4型约为200%。1型和2型黄体匀浆中E2的含量非常低，但3型增加到约200%，4型增加到约2500%。（摘要截断于250字）
