Kindya R J, Chan P C
Biochim Biophys Acta. 1976 Apr 8;429(2):608-15. doi: 10.1016/0005-2744(76)90309-0.
Human erythrocyte membrane fragments were exposed to O3 over varying lengths of time. Ozone was found to have a deleterious effect on the ouabainsensitive ATPase (EC 3.6.1.3) in the membrane fragments. After 1 min of exposure to O3, which was generated at a rate of 4.0 mumol/min, ouabain-sensitive ATPase activity decreased to 26% of the control. Ouabain-insensitive ATPase was found to be unaffected by O3 exposure under the test conditions. Additions of ascorbic acid or cysteine, prior to O3 exposure, partially protected the enzyme from inactivation. However, the inactivating effect of O3 could not be reversed by addition of either ascorbic acid or cysteine after exposure. Superoxide dismutase or catalase did not afford significant protection. The enzyme could not be protected by Ellman's reagent. The inactivating effect of O3 on the ouabain-sensitive ATPase was also demonstrated in exposure of intact erythrocytes. No detectable change was observed in glycolytic activity in the hemolysate prepared from O3-treated erythrocytes, however. It was postulated that inactivation of the membrane ATPase by O3 may be responsible for the destructive effect of O3 on the red cell.
将人红细胞膜片段暴露于臭氧中不同时长。发现臭氧对膜片段中的哇巴因敏感ATP酶（EC 3.6.1.3）具有有害作用。以4.0 μmol/分钟的速率产生臭氧，暴露1分钟后，哇巴因敏感ATP酶活性降至对照的26%。发现在测试条件下，哇巴因不敏感ATP酶不受臭氧暴露的影响。在臭氧暴露前添加抗坏血酸或半胱氨酸可部分保护该酶不被灭活。然而，暴露后添加抗坏血酸或半胱氨酸均不能逆转臭氧的灭活作用。超氧化物歧化酶或过氧化氢酶未提供显著保护。该酶不能被埃尔曼试剂保护。在完整红细胞的暴露实验中也证实了臭氧对哇巴因敏感ATP酶的灭活作用。然而，由臭氧处理过的红细胞制备的溶血产物中，糖酵解活性未观察到可检测到的变化。据推测，臭氧使膜ATP酶失活可能是臭氧对红细胞产生破坏作用的原因。
