一种温度敏感型CHO-K1细胞突变体（tsTM13），在M期染色体解聚和纺锤体解构方面存在缺陷。

A temperature-sensitive CHO-K1 cell mutant (tsTM13) defective in chromosome decondensation and spindle deconstruction in M phase.

作者信息

Tsuji H, Matsudo Y, Ajiro K, Yasuda H, Hanaoka F, Hayashi A, Utsumi S, Ohba Y, Hori T

机构信息

Division of Genetics, National Institute of Radiological Sciences, Chiba, Japan.

出版信息

Exp Cell Res. 1992 Feb;198(2):201-13. doi: 10.1016/0014-4827(92)90372-f.

DOI:10.1016/0014-4827(92)90372-f

PMID:1309502

Abstract

A temperature-sensitive CHO-K1 cell mutant, tsTM13, exhibited a delayed cell cycle progression from metaphase to telophase at a nonpermissive temperature and was finally arrested from anaphase to telophase. Metaphase chromosomes were overcondensed and chromosome disjunction in anaphase was uncoordinated. In telophase, sister chromatids were segregated and cytokinesis was completed, but chromosome structure remained in a condensed state and the spindle was not deconstructed. The level of phosphorylation of histones H1 and H3 remained high in the later stages of mitosis and the activity of histone H1 kinase was also maintained at a high level. These results strongly suggest that the pleiotropic defects of tsTM13 cells in mitosis are associated with a lack of inactivation of activated histone H1 kinase.

摘要

一种温度敏感型中国仓鼠卵巢细胞（CHO-K1）突变体tsTM13，在非允许温度下表现出从中期到末期的细胞周期进程延迟，最终从后期到末期停滞。中期染色体过度浓缩，后期染色体分离不协调。在末期，姐妹染色单体分离且胞质分裂完成，但染色体结构仍处于浓缩状态且纺锤体未解体。组蛋白H1和H3的磷酸化水平在有丝分裂后期仍保持较高，组蛋白H1激酶的活性也维持在较高水平。这些结果有力地表明，tsTM13细胞在有丝分裂中的多效性缺陷与活化的组蛋白H1激酶缺乏失活有关。