Clermont Y, Mauger A
Cell Tissue Kinet. 1976 Jan;9(1):99-104. doi: 10.1111/j.1365-2184.1976.tb01256.x.
Immature rats were used in an experiment to test the possible influence of a spermatogonial chalone on the expanding spermatogonial population in their developing testes. An extract from adult rat testes was injected intraperitoneally into 33-day-old rats and control animals were injected with an equal amount of saline. Two groups of normal adult rats similarly injected with the testicular extract and saline solutions served as additional controls. Following these injections, all animals were administered a dose of 3H-thymidine 10 hr before sacrifice. An analysis of the labeling indices of the various types of spermatogonia revealed that in young rats injected with testicular extract the percentage of labeled type A spermatogonia was significantly lower than in control animals. In contrast, the labeling indices of Intermediate and type B spermatogonia were similar in the two groups of young rats. In the two groups of adult animals, there was no difference in the labeling indices of type A or of other types of spermatogonia. These data indicated that the saline extract of adult testes contained a substance, a spermatogonial chalone, inhibiting specifically the proliferation of some type A spermatogonia. The results also support the concept that a spermatogonial chalone may intervene, through its action on the spermatogonial stem cell population, to arrest the growth of the seminiferous tubules as the animal reaches maturity.
在一项实验中使用未成熟大鼠来测试精原细胞抑素对其发育中睾丸内不断增加的精原细胞群体可能产生的影响。将成年大鼠睾丸提取物腹腔注射到33日龄大鼠体内，对照动物注射等量的生理盐水。两组同样注射了睾丸提取物和盐溶液的正常成年大鼠作为额外对照。这些注射后，所有动物在处死前10小时给予一剂3H-胸腺嘧啶核苷。对各种类型精原细胞的标记指数分析显示，注射睾丸提取物的幼鼠中，标记的A型精原细胞百分比显著低于对照动物。相反，两组幼鼠中中间型和B型精原细胞的标记指数相似。在两组成年动物中，A型或其他类型精原细胞的标记指数没有差异。这些数据表明成年睾丸的盐提取物含有一种物质，即精原细胞抑素，它特异性抑制某些A型精原细胞的增殖。结果还支持这样一种概念，即精原细胞抑素可能通过对精原干细胞群体的作用进行干预，在动物成熟时阻止生精小管的生长。
