铜离子（CuB）会是细胞色素c氧化酶中氧化还原连接的位点吗？
Could CuB be the site of redox linkage in cytochrome c oxidase?
作者信息
Larsen R W, Pan L P, Musser S M, Li Z Y, Chan S I
机构信息
Arthur Amos Noyes Laboratory of Chemical Physics, California Institute of Technology, Pasadena 91125.
出版信息
Proc Natl Acad Sci U S A. 1992 Jan 15;89(2):723-7. doi: 10.1073/pnas.89.2.723.
Abstract
This paper explores the proton pumping function of cytochrome c oxidase [ferrocytochrome-c:oxygen oxidoreductase (EC 1.9.3.1)] based upon redox linkage at the "high-potential" CuB center. A model is proposed that is derived from a redox-linked ligand exchange mechanism previously described for the CuA site. Qualitative analysis of this mechanism indicates that such a mechanism is feasible. However, the relatively short distance between CuB and cytochrome a3 implies that the uncoupling electron transfers are quite facile. In addition, the position of the CuB center with respect to the inner mitochondrial membrane argues against redox linkage at the CuB site.
摘要
本文基于“高电位”CuB中心的氧化还原连接，探讨了细胞色素c氧化酶[亚铁细胞色素c：氧氧化还原酶（EC 1.9.3.1）]的质子泵功能。提出了一个模型，该模型源自先前描述的CuA位点的氧化还原连接配体交换机制。对该机制的定性分析表明，这种机制是可行的。然而，CuB与细胞色素a3之间相对较短的距离意味着解偶联电子转移相当容易。此外，CuB中心相对于线粒体内膜的位置不利于在CuB位点进行氧化还原连接。
相似文献
1
Could CuB be the site of redox linkage in cytochrome c oxidase?铜离子（CuB）会是细胞色素c氧化酶中氧化还原连接的位点吗？
Proc Natl Acad Sci U S A. 1992 Jan 15;89(2):723-7. doi: 10.1073/pnas.89.2.723.
2
Cytochrome c oxidase: chemistry of a molecular machine.细胞色素c氧化酶：分子机器的化学原理
Adv Enzymol Relat Areas Mol Biol. 1995;71:79-208. doi: 10.1002/9780470123171.ch3.
3
The proton-pumping site of cytochrome c oxidase: a model of its structure and mechanism.细胞色素c氧化酶的质子泵浦位点：其结构与机制的模型
Biochim Biophys Acta. 1986;853(3-4):205-36. doi: 10.1016/0304-4173(87)90002-4.
4
The mechanism of transmembrane delta muH+ generation in mitochondria by cytochrome c oxidase.细胞色素c氧化酶在线粒体中产生跨膜质子动力势（delta muH+）的机制。
Biochem J. 1979 Jul 15;182(1):133-47. doi: 10.1042/bj1820133.
5
Identification of the electron transfers in cytochrome oxidase that are coupled to proton-pumping.鉴定细胞色素氧化酶中与质子泵浦相偶联的电子传递。
Nature. 1989 Apr 27;338(6218):776-8. doi: 10.1038/338776a0.
6
Time-resolved generation of membrane potential by ba cytochrome c oxidase from Thermus thermophilus coupled to single electron injection into the O and O states.时间分辨的热球菌细胞色素 c 氧化酶膜电位的产生与单个电子注入 O 和 O 态的耦合。
Biochim Biophys Acta Bioenerg. 2017 Nov;1858(11):915-926. doi: 10.1016/j.bbabio.2017.08.007. Epub 2017 Aug 12.
7
A proposal for the site and mechanism of redox-linked proton translocation in cytochrome c oxidase.细胞色素c氧化酶中氧化还原偶联质子转运的位点及机制的提议。
Prog Clin Biol Res. 1988;274:731-47.
8
The sequence of electron carriers in the reaction of cytochrome c oxidase with oxygen.细胞色素c氧化酶与氧气反应中电子载体的序列。
J Bioenerg Biomembr. 1993 Apr;25(2):115-20. doi: 10.1007/BF00762853.
9
Redox-linked proton translocation in cytochrome oxidase: the importance of gating electron flow. The effects of slip in a model transducer.细胞色素氧化酶中氧化还原相关的质子转运：控制电子流的重要性。模型换能器中滑移的影响。
Biophys J. 1986 Oct;50(4):713-33. doi: 10.1016/S0006-3495(86)83511-1.
10
Pumping of protons from the mitochondrial matrix by cytochrome oxidase.细胞色素氧化酶将质子从线粒体基质中泵出。
Nature. 1984;308(5959):558-60. doi: 10.1038/308558a0.
引用本文的文献
1
Modulation of the active site conformation by site-directed mutagenesis in cytochrome c oxidase from Paracoccus denitrificans.通过定向点突变对 Paracoccus denitrificans 细胞色素 c 氧化酶活性部位构象的调节。
J Inorg Biochem. 2010 Mar;104(3):318-23. doi: 10.1016/j.jinorgbio.2009.11.011. Epub 2009 Dec 3.
2
Spectroscopic evidence for a heme-heme binuclear center in the cytochrome bd ubiquinol oxidase from Escherichia coli.来自大肠杆菌的细胞色素bd泛醇氧化酶中血红素-血红素双核中心的光谱学证据。
Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5863-7. doi: 10.1073/pnas.90.12.5863.
3
Proton translocation in cytochrome c oxidase: redox linkage through proximal ligand exchange on cytochrome a3.细胞色素c氧化酶中的质子转运：通过细胞色素a3上近端配体交换实现的氧化还原连接。
J Bioenerg Biomembr. 1993 Apr;25(2):165-76. doi: 10.1007/BF00762858.
4
Insight into the active-site structure and function of cytochrome oxidase by analysis of site-directed mutants of bacterial cytochrome aa3 and cytochrome bo.通过分析细菌细胞色素aa3和细胞色素bo的定点突变体深入了解细胞色素氧化酶的活性位点结构与功能。
J Bioenerg Biomembr. 1993 Apr;25(2):121-36. doi: 10.1007/BF00762854.
5
Fluorescence quenching of reconstituted NCD-4-labeled cytochrome c oxidase complex by DOXYL-stearic acids.DOXYL-硬脂酸对重组NCD-4标记的细胞色素c氧化酶复合物的荧光猝灭作用。
Biophys J. 1993 Dec;65(6):2348-59. doi: 10.1016/S0006-3495(93)81309-2.
6
Mechanistic and phenomenological features of proton pumps in the respiratory chain of mitochondria.线粒体呼吸链中质子泵的机制和现象学特征
J Bioenerg Biomembr. 1994 Dec;26(6):609-18. doi: 10.1007/BF00831535.
7
The histidine cycle: a new model for proton translocation in the respiratory heme-copper oxidases.
J Bioenerg Biomembr. 1994 Dec;26(6):599-608. doi: 10.1007/BF00831534.
8
Understanding the cytochrome c oxidase proton pump: thermodynamics of redox linkage.了解细胞色素c氧化酶质子泵：氧化还原偶联的热力学
Biophys J. 1995 Jun;68(6):2543-55. doi: 10.1016/S0006-3495(95)80437-6.
本文引用的文献
1
Quaternary structures and low frequency molecular vibrations of haems of deoxy and oxyhaemoglobin studied by resonance raman scattering.通过共振拉曼散射研究脱氧血红蛋白和氧合血红蛋白血红素的四级结构及低频分子振动。
J Mol Biol. 1980 Jan 25;136(3):271-89. doi: 10.1016/0022-2836(80)90374-5.
2
Mechanism of free energy coupling in active transport.主动运输中自由能偶联的机制。
Annu Rev Biochem. 1983;52:379-409. doi: 10.1146/annurev.bi.52.070183.002115.
3
Energy-dependent reversal of the cytochrome oxidase reaction.细胞色素氧化酶反应的能量依赖性逆转
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4051-4. doi: 10.1073/pnas.78.7.4051.
4
Structural features and the reaction mechanism of cytochrome oxidase: iron and copper X-ray absorption fine structure.细胞色素氧化酶的结构特征与反应机制：铁和铜的X射线吸收精细结构
Biophys J. 1981 Jun;34(3):465-98. doi: 10.1016/S0006-3495(81)84863-1.
5
Spectroscopic characterization of compound C and related derivatives of cytochrome oxidase.细胞色素氧化酶的化合物C及相关衍生物的光谱表征
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1652-5. doi: 10.1073/pnas.78.3.1652.
6
Resonance Raman evidence for the activation of dioxygen in horseradish oxyperoxidase.
J Biol Chem. 1985 Jul 15;260(14):8372-7.
7
The proton-pumping site of cytochrome c oxidase: a model of its structure and mechanism.细胞色素c氧化酶的质子泵浦位点：其结构与机制的模型
Biochim Biophys Acta. 1986;853(3-4):205-36. doi: 10.1016/0304-4173(87)90002-4.
8
Redox-linked proton translocation in cytochrome oxidase: the importance of gating electron flow. The effects of slip in a model transducer.细胞色素氧化酶中氧化还原相关的质子转运：控制电子流的重要性。模型换能器中滑移的影响。
Biophys J. 1986 Oct;50(4):713-33. doi: 10.1016/S0006-3495(86)83511-1.
9
Modulation of cytochrome c oxidase activity by an electrical transmembrane gradient.
Ann N Y Acad Sci. 1988;550:269-76. doi: 10.1111/j.1749-6632.1988.tb35342.x.
10
The role of water near cytochrome a in cytochrome c oxidase.
Ann N Y Acad Sci. 1988;550:223-37. doi: 10.1111/j.1749-6632.1988.tb35338.x.
Zotero 插件