Herd J K, Forrest T, Tschida J
Clin Chim Acta. 1976 Apr 1;68(1):1-9. doi: 10.1016/0009-8981(76)90282-5.
Oligosaccharides of testicular hyaluronidase-degraded dermatan sulfate were separated from undegraded dermatan sulfate by chromatography on Sephadex G-75, but not by chromatography on Sephadex G-25. All but the smallest of these oligosaccharides were recovered in excellent yield following dialysis and precipitation with cetyl pyridinium chloride (CPC). G-75 chromatography of dialyzed, concentrated Hunter urine mucopolysaccharides precipitated with CPC resolved most of the large dermatan sulfate into a void volume related peak which was free of heparan sulfate. Decreasing amounts of dermatan sulfate oligosaccharides were eluted with sephadex-retarded polysaccharides, including small amounts which appeared with otherwise pure heparan sulfate.
通过在葡聚糖G - 75上进行色谱分析，可将睾丸透明质酸酶降解的硫酸皮肤素的寡糖与未降解的硫酸皮肤素分离，但在葡聚糖G - 25上进行色谱分析则无法分离。除了最小的这些寡糖外，其余寡糖在经过透析并用十六烷基氯化吡啶（CPC）沉淀后，均能以极高的产率回收。用CPC沉淀的透析浓缩亨特尿液黏多糖经G - 75色谱分析，可将大部分大分子硫酸皮肤素分离成一个与空体积相关的峰，该峰不含硫酸乙酰肝素。随着葡聚糖延迟多糖洗脱的硫酸皮肤素寡糖量逐渐减少，其中包括少量与其他纯净硫酸乙酰肝素一起出现的寡糖。
