猴水痘病毒和水痘带状疱疹病毒的基因组在大小和结构上相似。

The simian varicella virus and varicella zoster virus genomes are similar in size and structure.

作者信息

Gray W L, Pumphrey C Y, Ruyechan W T, Fletcher T M

机构信息

Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock 72205.

出版信息

Virology. 1992 Feb;186(2):562-72. doi: 10.1016/0042-6822(92)90022-h.

DOI:10.1016/0042-6822(92)90022-h

PMID:1310185

Abstract

Simian varicella virus (SVV) DNA was purified from viral nucleocapsids and the molecular structure of the SVV genome was determined. SVV DNA was analyzed by agarose gel electrophoresis of BamHI, BglII, EcoRI, and PstI restriction endonuclease digests. SVV and varicella zoster virus (VZV) DNAs were demonstrated to have distinct restriction endonuclease profiles. Summation of the sizes of individual restriction endonuclease fragments indicate the size of SVV DNA is congruent to 121 kilobase pairs (kbp) or congruent to 76.8 megadaltons (Md). Electron microscopy, lambda exonuclease analysis, and Southern blot DNA hybridizations were utilized to determine the molecular structure of the SVV genome and to construct restriction endonuclease maps. The results indicate that SVV DNA consists of a long component (L, congruent to 100 kbp) covalently linked to a short component (S, congruent to 20 kbp) which is composed of a unique short sequence (Us, 5.3 +/- 0.7 kbp) bracketed by inverted repeat sequences (TRs and IRs, congruent to 7.2 kbp). The presence of 0.5 M PstI restriction endonuclease fragments indicates that the S component may invert relative to the L component and that the genome exists in two major isomeric forms. The findings demonstrate that the SVV and VZV genomes are similar in size and structure.

摘要

从病毒核衣壳中纯化出猿猴水痘病毒（SVV）DNA，并确定了SVV基因组的分子结构。通过对BamHI、BglII、EcoRI和PstI限制性内切酶消化产物进行琼脂糖凝胶电泳分析SVV DNA。结果表明，SVV和水痘带状疱疹病毒（VZV）DNA具有不同的限制性内切酶图谱。对各个限制性内切酶片段大小的汇总表明，SVV DNA的大小相当于121千碱基对（kbp）或76.8兆道尔顿（Md）。利用电子显微镜、λ外切核酸酶分析和Southern印迹DNA杂交来确定SVV基因组的分子结构并构建限制性内切酶图谱。结果表明，SVV DNA由一个长片段（L，相当于100 kbp）和一个短片段（S，相当于20 kbp）共价连接而成，短片段由一个独特的短序列（Us，5.3±0.7 kbp）组成，两侧为反向重复序列（TRs和IRs，相当于7.2 kbp）。0.5 M PstI限制性内切酶片段的存在表明，S片段可能相对于L片段发生倒位，并且基因组以两种主要的异构体形式存在。这些发现表明，SVV和VZV基因组在大小和结构上相似。