Tominaga T, Yogo Y, Kitamura T, Aso Y
Department of Urology, Mitsui Memorial Hospital, Tokyo, Japan.
Virology. 1992 Feb;186(2):736-41. doi: 10.1016/0042-6822(92)90040-v.
JC virus DNAs derived from the urine of nonimmunosuppressed individuals generally contain an archetypal regulatory region which may have generated various regulatory regions of JC virus from from the brain with progressive multifocal leukoencephalopathy (PML). In this study, we examined whether JC virus persisting in normal human kidney tissue contains the archetypal regulatory region. Renal medulla, cortex, and tumor from 32 patients bearing renal tumors were screened for JC virus DNA by blot hybridization. Viral DNA was detected in the medulla in 13 cases (41%), in the cortex in 2 cases (6%), but not at all from the tumor. A number of viral DNA-positive specimens (8 from the medulla and 2 from the cortex) were used to amplify and sequence viral regulatory regions by polymerase chain reaction. Structures of the regulatory regions from all the specimens were, with a few nucleotide variations, identical with that of the archetypal region which was previously detected in the JC virus DNA from urine. This finding supports the hypothesis that the JC virus associated with PML evolved from the archetypal JC virus during persistence in human hosts. Furthermore, we present evidence that renal JCV is replicating and that progeny virions are excreted into the urine.
源自非免疫抑制个体尿液的JC病毒DNA通常含有一个原型调控区，该调控区可能在进行性多灶性白质脑病（PML）患者的大脑中产生了JC病毒的各种调控区。在本研究中，我们检测了存在于正常人类肾组织中的JC病毒是否含有原型调控区。通过印迹杂交对32例患有肾肿瘤患者的肾髓质、皮质和肿瘤进行JC病毒DNA筛查。在13例（41%）的髓质中检测到病毒DNA，在2例（6%）的皮质中检测到病毒DNA，但在肿瘤中未检测到。使用多个病毒DNA阳性标本（8个来自髓质，2个来自皮质）通过聚合酶链反应扩增并测序病毒调控区。所有标本调控区的结构，除了一些核苷酸变异外，与先前在尿液中的JC病毒DNA中检测到的原型区域相同。这一发现支持了与PML相关的JC病毒在人类宿主持续存在期间从原型JC病毒进化而来的假说。此外，我们提供了肾JCV正在复制且子代病毒颗粒被排泄到尿液中的证据。
