Takada N, Takase S, Enomoto N, Takada A, Date T
Department of Internal Medicine, Kanazawa Medical University, Ishikawa, Japan.
J Hepatol. 1992 Jan;14(1):35-40. doi: 10.1016/0168-8278(92)90128-c.
Hepatitis C virus (HCV) genomes were recently detected in biological materials, and variations of nucleotide sequences were reported. In the present study, typing of the HCV genomes was performed in 91 HCV-RNA-positive patients and the clinical features of patients with different types of HCV were compared. From the nucleotide sequences of the cDNA fragments, HCV can be divided into at least two types: HCV-K1-PT and HCV-K2. All cDNAs amplified from 91 patients were hybridized with cDNA probes of either HCV-K1-PT or HCV-K2. HCV-K1-PT was found in about 80% of the patients, and HCV-K2 was found in about 20% of the patients. These results indicate that types of HCV are limited to two types, i.e., K1-PT and K2, and the major type is HCV-K1-PT, at least in Japan. Detection rate of antibodies to C-100-3 protein were not different between the patients having HCV-K1-PT and HCV-K2, indicating that the antibodies may develop in HCV-related patients without relation to the types of the HCV genomes. Prevalence of the two types of HCV were nearly the same in various forms of NANB-related liver disease. However, the prevalence was somewhat different in alcoholic liver disease. HCV-K2 was found in patients younger than the patients with HCV-K1-PT. Frequency of a history of blood transfusion tended to be lower and the initial response to interferon treatment was clearly better in patients having HCV-K2 versus patients having HCV-K1-PT. These results suggest the possibility that clinical features due to HCV-K1 may be somewhat different from those due to HCV-K1-PT. However, the number of patients examined was too small to allow a definite conclusion, indicating a necessity for further study with a larger number of patients.
最近在生物材料中检测到丙型肝炎病毒（HCV）基因组，并报道了核苷酸序列的变异。在本研究中，对91例HCV-RNA阳性患者进行了HCV基因组分型，并比较了不同类型HCV患者的临床特征。根据cDNA片段的核苷酸序列，HCV可至少分为两种类型：HCV-K1-PT和HCV-K2。从91例患者中扩增的所有cDNA均与HCV-K1-PT或HCV-K2的cDNA探针杂交。在约80%的患者中发现了HCV-K1-PT，在约20%的患者中发现了HCV-K2。这些结果表明，HCV的类型限于两种，即K1-PT和K2，并且主要类型是HCV-K1-PT，至少在日本是这样。HCV-K1-PT和HCV-K2患者中抗C-100-3蛋白抗体的检测率没有差异，这表明抗体可能在HCV相关患者中产生，而与HCV基因组的类型无关。在各种形式的非甲非乙型（NANB）相关肝病中，两种类型HCV的流行率几乎相同。然而，在酒精性肝病中流行率有所不同。HCV-K2在比HCV-K1-PT患者更年轻的患者中被发现。与HCV-K1-PT患者相比，HCV-K2患者输血史的频率往往较低，并且对干扰素治疗的初始反应明显更好。这些结果提示，由HCV-K1引起的临床特征可能与由HCV-K1-PT引起的临床特征有所不同。然而，所检查的患者数量太少，无法得出明确结论，这表明有必要对更多患者进行进一步研究。
