Rimell F, Maisel R, Dayton V
Department of Otolaryngology, University of Minnesota, Minneapolis.
Ann Otol Rhinol Laryngol. 1992 Feb;101(2 Pt 1):119-26. doi: 10.1177/000348949210100203.
The technique of in situ hybridization with biotin-labeled probes was applied to 20 patients with laryngeal papillomatosis (9 with adult-onset disease and 11 with juvenile-onset). Ten patients were noted for epithelial transformation of their laryngeal disease. All patients, regardless of their clinical outcome, tested positive for human papillomavirus group 6/11. The biotin method of in situ hybridization proved to be a sensitive method in this study, identifying the infecting viral group in 23 of 24 samples. This study also tested for group 16/18 and is the first to test for the presence of group 31/33/35 in the larynx. These viruses or members of related groups have been reported in malignancies of the head and neck as well as the uterine cervix. They have yet, however, to be identified in benign laryngeal papillomas or laryngeal papillomas that have undergone epithelial transformation.
将生物素标记探针原位杂交技术应用于20例喉乳头状瘤患者（9例成人发病，11例青少年发病）。10例患者出现喉部疾病上皮化生。所有患者，无论其临床结局如何，人乳头瘤病毒6/11组检测均为阳性。在本研究中，生物素原位杂交方法被证明是一种敏感方法，在24个样本中的23个中识别出感染病毒组。本研究还检测了16/18组，并且是首个检测喉部是否存在31/33/35组的研究。这些病毒或相关组的成员已在头颈部以及子宫颈恶性肿瘤中被报道。然而，它们尚未在良性喉乳头状瘤或已发生上皮化生的喉乳头状瘤中被识别出来。
