免疫复合物性肾炎中的抗原。V. 通过凝胶沉淀法进行回收与鉴定
Antigen in immune complex nephritis. V. Recovery and identification by gel precipitation.
作者信息
Milgrom F, Campbell W A, Andres G A
出版信息
Immunology. 1976 Feb;30(2):277-80.
Abstract
A procedure is described for detection of antibodies and antigens in 'immune complex' nephritis. Kidney tissue is minced, washed and sealed in a well of an agarose or agar plate. Separation of immune complexes is achieved by electrophoresis at 56 degrees. Afterwards, antibody is readily detected in reaction with a proper anti-IgG serum. Antigen is identified by its corresponding antibody, the reaction with which is, in some instances, strengthened by counterimmunoelectrophoresis.
摘要
本文描述了一种检测“免疫复合物”性肾炎中抗体和抗原的方法。将肾组织切碎、洗涤，然后封入琼脂糖或琼脂平板的孔中。通过在56摄氏度下进行电泳来分离免疫复合物。之后，用适当的抗IgG血清反应可容易地检测到抗体。抗原通过其相应的抗体来鉴定，在某些情况下，通过对流免疫电泳可增强其与抗体的反应。
Zotero 插件