Holloway Y, Snijder J A, Boersma W G
Immunology Research, Regional Public Health Laboratory, Groningen, The Netherlands.
J Clin Microbiol. 1993 Dec;31(12):3247-54. doi: 10.1128/jcm.31.12.3247-3254.1993.
An enzyme-linked immunosorbent assay was developed for quantitation of circulating immune complexes (CICs) containing specific antipneumococcal immunoglobulin G (IgG). These CICs were detected in 17 (85%) of 20 patients with bacteremic pneumococcal pneumonia, 4 (36.4%) of 11 patients with probable pneumococcal pneumonia, 3 (16.7%) of 18 patients with pneumonia of other (nonpneumococcal) etiology, and 13 (41.9%) of 31 patients with pneumonia of unknown etiology. There was no correlation between CICs and serum IgG antibody levels. Pneumococcal capsular antigen was demonstrated in dissociated CICs by latex agglutination.
开发了一种酶联免疫吸附测定法,用于定量检测含有特异性抗肺炎球菌免疫球蛋白G(IgG)的循环免疫复合物(CIC)。在20例菌血症性肺炎球菌肺炎患者中的17例(85%)、11例可能为肺炎球菌肺炎患者中的4例(36.4%)、18例其他(非肺炎球菌)病因的肺炎患者中的3例(16.7%)以及31例病因不明的肺炎患者中的13例(41.9%)检测到了这些CIC。CIC与血清IgG抗体水平之间无相关性。通过乳胶凝集试验在解离的CIC中检测到肺炎球菌荚膜抗原。