Maione R, Fimia G M, Amati P
Dipartimento di Biopatologia Umana, Università di Roma La Sapienza, Italy.
Oncogene. 1992 Jan;7(1):85-93.
In the present work we report on the role of a polyomavirus (Py) early function in interfering with both morphological and biochemical differentiation of the myogenic C2 cell line. The analysis of cell clones stably transfected with a plasmid carrying an ORI- Py genome showed that in the presence of the whole viral early region myogenesis is blocked and a transformed phenotype is evident. By using a plasmid that only encodes large-T function, the involvement of this individual early viral gene product was determined. Inhibition of myogenic differentiation by Py large T is proportional to the level of its expression. This inhibition does not appear to require alteration of cell growth properties. The analysis of muscle-specific functions expressed at different steps in the myogenic pathway showed that Py large T blocks the expression of terminal differentiation markers without altering the expression of the regulatory gene MyoD.
在本研究中，我们报告了一种多瘤病毒（Py）早期功能在干扰成肌C2细胞系的形态和生化分化方面的作用。对稳定转染携带ORI-Py基因组质粒的细胞克隆进行分析表明，在整个病毒早期区域存在的情况下，肌生成被阻断，转化表型明显。通过使用仅编码大T功能的质粒，确定了这种单个早期病毒基因产物的作用。Py大T对成肌分化的抑制作用与其表达水平成正比。这种抑制作用似乎不需要改变细胞生长特性。对成肌途径不同步骤表达的肌肉特异性功能进行分析表明，Py大T阻断终末分化标志物的表达，而不改变调节基因MyoD的表达。
