Enkemann S A, Konieczny S F, Taparowsky E J
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
Cell Growth Differ. 1990 Aug;1(8):375-82.
The early region 1A of adenoviruses encodes two multifunctional proteins that have varied effects on viral and host cell transcription. Expression of adenovirus 5 E1A in the mouse myogenic cell line 23A2 inhibits the differentiation of the cells and represses the expression of reporter genes controlled by several muscle-specific regulatory elements. In addition, differentiation-defective 23A2 E1A cells do not express MyoD1 or myogenin, the two myogenic regulatory factors that normally are expressed at high levels in this cell line. We conclude that E1A exerts a negative effect on the differentiation of skeletal muscle cells by interfering with the expression of myogenic regulatory factor genes that are essential for the transcriptional activation of the contractile protein gene set.
腺病毒的早期区域1A编码两种多功能蛋白，它们对病毒和宿主细胞转录有不同影响。在小鼠成肌细胞系23A2中表达腺病毒5 E1A可抑制细胞分化，并抑制由几种肌肉特异性调控元件控制的报告基因的表达。此外，分化缺陷的23A2 E1A细胞不表达MyoD1或肌细胞生成素，这两种成肌调节因子通常在该细胞系中高水平表达。我们得出结论，E1A通过干扰对收缩蛋白基因集转录激活至关重要的成肌调节因子基因的表达，对骨骼肌细胞的分化产生负面影响。
