Mitchell P G, Struve J A, McCarthy G M, Cheung H S
Department of Medicine, Medical College of Wisconsin, Milwaukee 53226.
Arthritis Rheum. 1992 Mar;35(3):343-50. doi: 10.1002/art.1780350314.
To investigate BCP (basic calcium phosphate) crystal-stimulated mitogenesis and collagenase gene transcription in primary cultures of porcine chondrocytes.
The role of protein kinases in BCP crystal-stimulated DNA synthesis was investigated using thymidine incorporation, kinase inhibitors, and protein kinase C (PKC) assays. Northern blot analysis was used to determine the levels of collagenase c-fos and c-jun message.
BCP crystals stimulated chondrocyte proliferation in a PKC-dependent manner. Increased levels of collagenase message were preceded by an increased accumulation of c-fos, but not c-jun.
BCP crystals could contribute to the abnormal chondrocyte proliferation and collagenase secretion observed in some rheumatic diseases.
研究碱性磷酸钙（BCP）晶体刺激猪软骨细胞原代培养物中的有丝分裂和胶原酶基因转录。
使用胸腺嘧啶核苷掺入法、激酶抑制剂和蛋白激酶C（PKC）测定法研究蛋白激酶在BCP晶体刺激的DNA合成中的作用。采用Northern印迹分析来确定胶原酶、c-fos和c-jun信息的水平。
BCP晶体以PKC依赖的方式刺激软骨细胞增殖。在胶原酶信息水平升高之前，c-fos的积累增加，但c-jun没有增加。
BCP晶体可能导致某些风湿性疾病中观察到的软骨细胞异常增殖和胶原酶分泌。
