Lehtola L, Partanen J, Sistonen L, Korhonen J, Wärri A, Härkönen P, Clarke R, Alitalo K
Department of Pathology, University of Helsinki, Finland.
Int J Cancer. 1992 Feb 20;50(4):598-603. doi: 10.1002/ijc.2910500419.
The MCF-7 cell line is a hormone-responsive human breast-cancer cell line, which has been extensively used in studies of estrogen regulation of cell growth. These studies have indicated that the growth stimulation of the MCF-7 cells by estrogens may be effected by an autocrine mechanism involving several growth factors, such as EGF, TGF alpha and IGF-I and their receptors. We have amplified and cloned tyrosine-kinase-related sequences from the MCF-7 cell mRNA using the polymerase chain reaction and characterized the partial cDNAs obtained by nucleic acid sequencing. Nine tyrosine kinase cDNAs and one serine/threonine kinase cDNA were identified among the amplified sequences. Four different tyrosine kinase genes encoding receptors for fibroblast growth factors (FGFs) were found to be expressed by the MCF-7 cells. In addition, differences were observed in the expression of these members of FGF receptor family in different breast-cancer cells. A putative tyrosine-kinase receptor and a novel serine/threonine kinase were preferentially expressed in estrogen-responsive tumor cell lines. However, no estrogen-dependent regulation of any of the novel tyrosine-kinase receptor mRNAs was found in any of the cell lines including the MCF-7 or ZR-75-I cells, where the expression of the neu proto-oncogene mRNA was decreased during estrogen treatment. The expression of several FGF receptors by breast-cancer cells suggests that FGFs may be involved in their growth regulation and tumorigenesis.
MCF - 7细胞系是一种激素反应性人乳腺癌细胞系，已广泛用于雌激素对细胞生长调节的研究。这些研究表明，雌激素对MCF - 7细胞的生长刺激可能通过一种自分泌机制实现，该机制涉及多种生长因子，如表皮生长因子（EGF）、转化生长因子α（TGFα）和胰岛素样生长因子 - I（IGF - I）及其受体。我们利用聚合酶链反应从MCF - 7细胞mRNA中扩增并克隆了酪氨酸激酶相关序列，并通过核酸测序对获得的部分cDNA进行了表征。在扩增序列中鉴定出9个酪氨酸激酶cDNA和1个丝氨酸/苏氨酸激酶cDNA。发现MCF - 7细胞表达4种不同的编码成纤维细胞生长因子（FGFs）受体的酪氨酸激酶基因。此外，在不同的乳腺癌细胞中观察到FGF受体家族这些成员的表达存在差异。一种推定的酪氨酸激酶受体和一种新型丝氨酸/苏氨酸激酶在雌激素反应性肿瘤细胞系中优先表达。然而，在包括MCF - 7或ZR - 75 - I细胞在内的任何细胞系中，均未发现任何新型酪氨酸激酶受体mRNA的雌激素依赖性调节，在雌激素处理期间，这些细胞系中neu原癌基因mRNA的表达会降低。乳腺癌细胞对几种FGF受体的表达表明，FGFs可能参与其生长调节和肿瘤发生。
