用重组DNA编码的禽冠状病毒核衣壳蛋白免疫诱导抗病毒免疫反应。
Induction of anti-viral immune responses by immunization with recombinant-DNA encoded avian coronavirus nucleocapsid protein.
作者信息
Boots A M, Benaissa-Trouw B J, Hesselink W, Rijke E, Schrier C, Hensen E J
机构信息
Department of Immunology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.
出版信息
Vaccine. 1992;10(2):119-24. doi: 10.1016/0264-410x(92)90028-i.
Abstract
Immune responses to the infectious bronchitis virus (IBV) nucleocapsid protein were studied using a recombinant-DNA expression product. In mice, a lymphocyte proliferative response and a delayed-type hypersensitivity reaction to IBV were induced upon immunization with this nucleocapsid protein. Next, we studied the role of the expressed nucleocapsid protein in induction of a protective immune response to IBV in chickens. Chickens were primed with nucleocapsid protein and subsequently boosted with inactivated IBV, strain M41. Proliferative responses of blood mononuclear cells corresponded with increased mean haemagglutination inhibition and virus neutralization titres. Finally, an increased tracheal protection against challenge with live IBV was observed. These results indicate that infectious bronchitis virus nucleocapsid protein is a relevant target for immune recognition in both the mouse and the chicken.
摘要
利用重组 DNA 表达产物研究了对传染性支气管炎病毒（IBV）核衣壳蛋白的免疫反应。在用该核衣壳蛋白免疫小鼠后，诱导了对 IBV 的淋巴细胞增殖反应和迟发型超敏反应。接下来，我们研究了表达的核衣壳蛋白在诱导鸡对 IBV 的保护性免疫反应中的作用。用核衣壳蛋白对鸡进行初次免疫，随后用灭活的 M41 株 IBV 进行加强免疫。血液单核细胞的增殖反应与平均血凝抑制和病毒中和效价的增加相对应。最后，观察到气管对活 IBV 攻击的保护作用增强。这些结果表明，传染性支气管炎病毒核衣壳蛋白是小鼠和鸡免疫识别的相关靶点。
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