Key Laboratory of Animal Vaccine Development, Ministry of Agriculture, Guangzhou 510642, China; College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Huizhou Entry-Exit Inspection and Quarantine Bureau, Huizhou 516001, China.
Vaccine. 2014 Feb 3;32(6):664-70. doi: 10.1016/j.vaccine.2013.12.006. Epub 2013 Dec 14.
Avian infectious bronchitis virus (IBV) is associated with production inefficiencies in domestic fowl, and causes massive economic losses to the poultry industry worldwide. Progress has been made in designing novel and efficient candidate vaccines to control IBV infection. BacMam virus, a modified baculovirus mediating transgene expression under the control of a mammalian promoter, has emerged as a versatile and safe vector during vaccine development. In previous work, we generated the BacMam virus Ac-CMV-S1, which expressed the S1 glycoprotein of IBV-M41. We showed that Ac-CMV-S1 induced excellent cellular immunity, but did not confer adequate protection in chickens compared with the conventional inactivated vaccine. In the current study, we generated an improved BacMam virus, BV-Dual-S1. This virus displayed the S1 glycoprotein on the baculovirus envelope, and was capable of expressing it in mammalian cells. BV-Dual-S1 elicited stronger humoral and cell-mediated immune responses, and showed greater capacity for induction of cytotoxic T lymphocyte responses, compared with Ac-CMV-S1 in specific pathogen-free chickens. A significant difference was not observed for protection rates between chickens immunized with BV-Dual-S1 (83%) or inactivated vaccine (89%) following challenge with virulent IBV-M41. Our findings show that the protective efficacy of BV-Dual-S1 could be significantly enhanced by baculovirus display technology. BacMam virus-based surface display strategies could serve as effective tools in designing vaccines against IB and other infectious diseases.
禽传染性支气管炎病毒（IBV）与家禽生产效率低下有关，给全球家禽业造成了巨大的经济损失。在设计新型、高效的候选疫苗以控制 IBV 感染方面已经取得了进展。BacMam 病毒是一种经过修饰的杆状病毒，在哺乳动物启动子的控制下介导转基因表达，它在疫苗开发过程中已成为一种多功能且安全的载体。在之前的工作中，我们生成了 BacMam 病毒 Ac-CMV-S1，它表达了 IBV-M41 的 S1 糖蛋白。我们发现 Ac-CMV-S1 诱导了出色的细胞免疫，但与传统的灭活疫苗相比，在鸡中并未提供足够的保护。在当前的研究中，我们生成了一种改良的 BacMam 病毒 BV-Dual-S1。该病毒在杆状病毒包膜上展示了 S1 糖蛋白，并能够在哺乳动物细胞中表达它。与 Ac-CMV-S1 相比，BV-Dual-S1 在无特定病原体鸡中引起了更强的体液和细胞介导的免疫反应，并且显示出诱导细胞毒性 T 淋巴细胞反应的更大能力。用 BV-Dual-S1（83%）或灭活疫苗（89%）免疫的鸡在受到强毒 IBV-M41 攻击后的保护率没有显著差异。我们的研究结果表明，杆状病毒展示技术可以显著增强 BV-Dual-S1 的保护效力。BacMam 病毒为基础的表面展示策略可作为设计针对 IB 和其他传染病疫苗的有效工具。
