Margolin Y, Behrman H R
Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, Connecticut 06510.
Am J Physiol. 1992 Feb;262(2 Pt 1):E173-8. doi: 10.1152/ajpendo.1992.262.2.E173.
The production of reactive oxygen species in the ovary is rapidly inducible, but the nature of the generator is unknown. One possibility is xanthine oxidase (XO), an enzyme that produces superoxide in the presence of hypoxanthine (or xanthine) and oxygen. The objective of the present studies was to measure levels of XO in follicular and luteal tissue to determine whether XO may be a source of reactive oxygen species in the rat ovary. Ovarian levels of XO were about one-fifth of that seen in the liver and adrenal, and XO levels were about one-third of xanthine dehydrogenase (XDH). Preovulatory ovarian levels of XO activity were unchanged after induction of ovulation with gonadotropin and in follicles incubated with gonadotropin. Luteal XO activity was not changed during natural or prostaglandin F2 alpha (PGF2 alpha)-induced luteolysis. Allopurinol, an inhibitor of XO, did not inhibit ovulation or PGF2 alpha-induced luteal regression. Finally, neither catalase and superoxide dismutase nor oxypurinol altered luteal cell function in the presence of hypoxanthine. Thus, while XO is present in the ovary, it does not appear that it is a major source of reactive oxygen species in this organ.
卵巢中活性氧的产生可迅速被诱导，但产生活性氧的物质本质尚不清楚。一种可能是黄嘌呤氧化酶（XO），该酶在次黄嘌呤（或黄嘌呤）和氧气存在的情况下产生超氧化物。本研究的目的是测量卵泡和黄体组织中XO的水平，以确定XO是否可能是大鼠卵巢中活性氧的来源。卵巢中XO的水平约为肝脏和肾上腺中XO水平的五分之一，且XO水平约为黄嘌呤脱氢酶（XDH）水平的三分之一。用促性腺激素诱导排卵后，以及在与促性腺激素一起孵育的卵泡中，排卵前卵巢的XO活性没有变化。在自然黄体溶解或前列腺素F2α（PGF2α）诱导的黄体溶解过程中，黄体的XO活性没有改变。XO抑制剂别嘌呤醇不抑制排卵或PGF2α诱导的黄体退化。最后，在次黄嘌呤存在的情况下，过氧化氢酶、超氧化物歧化酶和氧嘌呤醇均未改变黄体细胞功能。因此，虽然卵巢中存在XO，但它似乎并不是该器官中活性氧的主要来源。
