Tijburg L B, Vermeulen P S, Schmitz M G, van Golde L M
Laboratory of Veterinary Biochemistry, Utrecht University, The Netherlands.
Biochem Biophys Res Commun. 1992 Feb 14;182(3):1226-31. doi: 10.1016/0006-291x(92)91862-k.
Okadaic acid, a specific inhibitor of protein phosphatase 1 and 2A, inhibited the synthesis of phosphatidylethanolamine via the CDPethanolamine pathway in isolated hepatocytes. Pulse-chase experiments and measurement of the enzyme activity demonstrated that the inhibition of phosphatidylethanolamine synthesis was not caused by an inhibition of CTP:phosphoethanolamine cytidylyltransferase, the putative regulatory enzyme. However, okadaic acid decreased the cellular diacylglycerol level to 30% of that in control cells. The data suggest that the availability of diacylglycerol limits phosphatidylethanolamine synthesis in okadaic acid-treated hepatocytes.
冈田酸是蛋白磷酸酶1和2A的特异性抑制剂，它在分离的肝细胞中通过CDP - 乙醇胺途径抑制磷脂酰乙醇胺的合成。脉冲追踪实验和酶活性测定表明，磷脂酰乙醇胺合成的抑制并非由假定的调节酶CTP：磷酸乙醇胺胞苷转移酶的抑制所致。然而，冈田酸使细胞二酰甘油水平降至对照细胞的30%。数据表明，在经冈田酸处理的肝细胞中，二酰甘油的可利用性限制了磷脂酰乙醇胺的合成。
