胰高血糖素对离体大鼠肝细胞中磷脂酰乙醇胺合成的抑制作用。
Inhibition of phosphatidylethanolamine synthesis by glucagon in isolated rat hepatocytes.
作者信息
Tijburg L B, Houweling M, Geelen M J, Van Golde L M
机构信息
Laboratory of Veterinary Biochemistry, University of Utrecht, The Netherlands.
出版信息
Biochem J. 1989 Feb 1;257(3):645-50. doi: 10.1042/bj2570645.
Abstract
Exposure of isolated rat hepatocytes to glucagon or chlorophenylthio cyclic AMP led to an inhibition of the incorporation of [1,2-14C]ethanolamine into phosphatidylethanolamine. Pulse-chase experiments and measurement of the activities of the enzymes involved in the CDP-ethanolamine pathway provided evidence that the inhibitory effect of glucagon on the synthesis de novo of phosphatidylethanolamine was not caused by a diminished conversion of ethanolamine phosphate into CDP-ethanolamine. The observations suggested that the glucagon-induced inhibition of the biosynthesis of phosphatidylethanolamine is probably due to a decreased supply of diacylglycerols, resulting in a decreased formation of phosphatidylethanolamine from CDP-ethanolamine and diacylglycerols.
摘要
将分离的大鼠肝细胞暴露于胰高血糖素或氯苯硫基环磷酸腺苷会导致[1,2-14C]乙醇胺掺入磷脂酰乙醇胺受到抑制。脉冲追踪实验以及对参与CDP-乙醇胺途径的酶活性的测量提供了证据，表明胰高血糖素对磷脂酰乙醇胺从头合成的抑制作用并非由磷酸乙醇胺向CDP-乙醇胺的转化减少所致。这些观察结果表明，胰高血糖素诱导的磷脂酰乙醇胺生物合成抑制可能是由于二酰基甘油供应减少，导致由CDP-乙醇胺和二酰基甘油形成的磷脂酰乙醇胺减少。
相似文献
1
Inhibition of phosphatidylethanolamine synthesis by glucagon in isolated rat hepatocytes.
Biochem J. 1989 Feb 1;257(3):645-50. doi: 10.1042/bj2570645.
2
Biosynthesis of phosphatidylethanolamine via the CDP-ethanolamine route is an important pathway in isolated rat hepatocytes.
Biochem Biophys Res Commun. 1989 May 15;160(3):1275-80. doi: 10.1016/s0006-291x(89)80141-x.
3
Stimulation of phosphatidylethanolamine synthesis in isolated rat hepatocytes by phorbol 12-myristate 13-acetate.
Biochim Biophys Acta. 1987 Nov 21;922(2):184-90. doi: 10.1016/0005-2760(87)90153-6.
4
Okadaic acid inhibits phosphatidylethanolamine biosynthesis in rat hepatocytes.
Biochem Biophys Res Commun. 1992 Feb 14;182(3):1226-31. doi: 10.1016/0006-291x(92)91862-k.
5
Synthesis of phosphatidylcholines in rat hepatocytes. Possible regulation by norepinephrine via an alpha-adrenergic mechanism.
J Biol Chem. 1984 Sep 25;259(18):11273-8.
6
Regulation of phospholipid biosynthesis in isolated rat hepatocytes. Effect of different substrates.
J Biol Chem. 1975 May 10;250(9):3359-67.
7
Phosphatidylethanolamine metabolism in rat liver after partial hepatectomy. Control of biosynthesis of phosphatidylethanolamine by the availability of ethanolamine.
Biochem J. 1992 Apr 1;283 ( Pt 1)(Pt 1):55-61. doi: 10.1042/bj2830055.
8
Effects of vasopressin on the synthesis of phosphatidylethanolamines and phosphatidylcholines by isolated rat hepatocytes.
Biochim Biophys Acta. 1987 May 13;919(1):49-57. doi: 10.1016/0005-2760(87)90216-5.
9
Choline regulates phosphatidylethanolamine biosynthesis in isolated hamster heart.
J Biol Chem. 1982 Nov 25;257(22):13201-4.
10
The effect of cyclic AMP analogues and glucagon on cholesteryl ester synthesis and hydrolysis in cultured hamster hepatocytes.
FEBS Lett. 1993 Aug 23;329(1-2):17-20. doi: 10.1016/0014-5793(93)80183-u.
引用本文的文献
1
Significance of bilayer-forming phospholipids for skeletal muscle insulin sensitivity and mitochondrial function.
J Biomed Res. 2019 May 31;34(1):1-13. doi: 10.7555/JBR.33.20180104.
2
Historical perspective: phosphatidylserine and phosphatidylethanolamine from the 1800s to the present.
J Lipid Res. 2018 Jun;59(6):923-944. doi: 10.1194/jlr.R084004. Epub 2018 Apr 16.
3
The development of a metabolic disease phenotype in CTP:phosphoethanolamine cytidylyltransferase-deficient mice.
J Biol Chem. 2009 Sep 18;284(38):25704-13. doi: 10.1074/jbc.M109.023846. Epub 2009 Jul 22.
4
Cloning and expression of a human choline/ethanolaminephosphotransferase: synthesis of phosphatidylcholine and phosphatidylethanolamine.
Biochem J. 1999 Apr 15;339 ( Pt 2)(Pt 2):291-8.
5
Subcellular distribution of agonist-stimulated phosphatidylinositol synthesis in 1321 N1 astrocytoma cells.
Biochem J. 1993 Mar 1;290 ( Pt 2)(Pt 2):381-7. doi: 10.1042/bj2900381.
6
Modulation of biliary lipid secretion by forskolin and cyclic AMP analogues.
Biochem J. 1990 Feb 1;265(3):879-85. doi: 10.1042/bj2650879.
7
Phosphatidylinositol synthase and phosphatidylinositol/inositol exchange reactions in turkey erythrocyte membranes.
Biochem J. 1991 Apr 1;275 ( Pt 1)(Pt 1):187-92. doi: 10.1042/bj2750187.
8
Phosphatidylcholine metabolism in rat liver after partial hepatectomy. Evidence for increased activity and amount of CTP:phosphocholine cytidylyltransferase.
Biochem J. 1991 Sep 1;278 ( Pt 2)(Pt 2):347-51. doi: 10.1042/bj2780347.
9
Phosphatidylethanolamine metabolism in rat liver after partial hepatectomy. Control of biosynthesis of phosphatidylethanolamine by the availability of ethanolamine.
Biochem J. 1992 Apr 1;283 ( Pt 1)(Pt 1):55-61. doi: 10.1042/bj2830055.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75.
2
Glucagon inhibits phosphatidylcholine biosynthesis via the CDP-choline and transmethylation pathways in cultured rat hepatocytes.
Can J Biochem Cell Biol. 1984 Apr;62(4):196-202. doi: 10.1139/o84-028.
3
Regulation of phosphatidylcholine biosynthesis.
Biochim Biophys Acta. 1984 Jun 25;779(2):217-51. doi: 10.1016/0304-4157(84)90010-8.
4
Regulation of rat liver cytosolic CTP: phosphocholine cytidylyltransferase by phosphorylation and dephosphorylation.
J Biol Chem. 1982 Dec 10;257(23):14198-202.
5
Analogues of cyclic AMP inhibit phosphatidylethanolamine N-methylation by cultured rat hepatocytes.
Biochim Biophys Acta. 1981 Nov 23;666(2):301-6. doi: 10.1016/0005-2760(81)90122-3.
6
Prolonged effects of cyclic AMP analogues of phosphatidylcholine biosynthesis in cultured rat hepatocytes.
Biochim Biophys Acta. 1982 Nov 12;713(2):260-9. doi: 10.1016/0005-2760(82)90243-0.
7
cAMP analogues inhibit phosphatidylcholine biosynthesis in cultured rat hepatocytes.
J Biol Chem. 1981 Aug 25;256(16):8283-6.
8
Short-term hormonal control of hepatic lipogenesis.
Diabetes. 1980 Dec;29(12):1006-22. doi: 10.2337/diab.29.12.1006.
9
Can phosphorylation of phosphatidate phosphohydrolase by a cyclic AMP-dependent mechanism regulate its activity and subcellular distribution and control hepatic glycerolipid synthesis?
Biochem J. 1984 Sep 1;222(2):487-93. doi: 10.1042/bj2220487.
10
Effect of different fatty acids on glycerolipid synthesis in isolated rat hepatocytes.
J Biol Chem. 1974 Aug 25;249(16):5102-7.
Zotero 插件