Kobayashi H, Fukata J, Tominaga T, Murakami N, Fukushima M, Ebisui O, Segawa H, Nakai Y, Imura H
Department of Internal Medicine, Kyoto University Faculty of Medicine, Japan.
FEBS Lett. 1992 Feb 17;298(1):100-4. doi: 10.1016/0014-5793(92)80031-b.
To study the cellular mechanisms of interleukin-1 (IL-1) in the pituitary corticotroph, we studied the properties of IL-1 receptors on a mouse pituitary ACTH-producing cell line, AtT-20. Scatchard plot analysis revealed a single type of receptor with a Kd (dissociation constant) of 93 pM, and 482 binding sites/cell. [125I]IL-1 alpha binding competed with IL-1 alpha and IL-1 beta in an equimolar fashion. A 24 h pre-incubation with either CRH, epinephrine or nor-epinephrine increased the [125I]IL-1 alpha binding sites in the AtT-20 cells and conversely, a similar pre-incubation with either dexamethasone or tumour necrosis factor-alpha (TNF alpha) decreased them without affecting the affinity of the receptors in either case.
为研究白细胞介素-1（IL-1）在垂体促肾上腺皮质激素细胞中的细胞机制，我们研究了小鼠垂体促肾上腺皮质激素分泌细胞系AtT-20上IL-1受体的特性。Scatchard作图分析显示存在一种单一类型的受体，其解离常数（Kd）为93 pM，每个细胞有482个结合位点。[125I]IL-1α结合以等摩尔方式与IL-1α和IL-1β竞争。用促肾上腺皮质激素释放激素（CRH）、肾上腺素或去甲肾上腺素进行24小时预孵育可增加AtT-20细胞中[125I]IL-1α结合位点，相反，用地塞米松或肿瘤坏死因子-α（TNFα）进行类似的预孵育可减少这些位点，且在两种情况下均不影响受体的亲和力。
