Bristulf J, Simoncsits A, Bartfai T
Department of Biochemistry, Arrheniuslaboratory, Stockholm University, Sweden.
Neurosci Lett. 1991 Jul 22;128(2):173-6. doi: 10.1016/0304-3940(91)90254-q.
Interleukin-1 (IL-1) mediates numerous responses on the mouse anterior pituitary cell line AtT-20. We have studied the ligand binding properties of the IL-1 receptors (IL-1R) of the AtT-20 cells and found that they possess 1.5 x 10(3) receptors/cell with a Kd of 0.15 nM for [125I]IL-1 alpha. Using oligonucleotide primers, which define a 236 bp region of the cDNA coding for the cytosolic part of the mouse T-cell IL-1R, and cDNA prepared from AtT-20 cells, we obtained by polymerase chain reaction (PCR) a DNA fragment which was shown to possess an identical sequence to that of the corresponding region of the mouse T-cell IL-1R. Thus the AtT-20 pituitary cell line IL-1 receptor appears to be similar to those found on mouse T-cells and fibroblasts.
白细胞介素-1（IL-1）介导对小鼠垂体前叶细胞系AtT-20的多种反应。我们研究了AtT-20细胞的IL-1受体（IL-1R）的配体结合特性，发现它们具有1.5×10³个受体/细胞，对[¹²⁵I]IL-1α的解离常数（Kd）为0.15 nM。使用限定小鼠T细胞IL-1R胞质部分编码cDNA的236 bp区域的寡核苷酸引物，以及从AtT-20细胞制备的cDNA，我们通过聚合酶链反应（PCR）获得了一个DNA片段，该片段显示与小鼠T细胞IL-1R相应区域的序列相同。因此，AtT-20垂体细胞系的IL-1受体似乎与在小鼠T细胞和成纤维细胞上发现的受体相似。
