Párraga A, Portugal J
Departamento de Bioquímica y Fisiologia, Universidad de Barcelona, Spain.
FEBS Lett. 1992 Mar 23;300(1):25-9. doi: 10.1016/0014-5793(92)80157-c.
The sequence specificity of elsamicin A, an anti-tumour antibiotic, binding to DNA was elucidated considering the inhibition of the rate of digestion of linearised pBR322 DNA by AatII, ClaI, EcoRI, HindIII and NruI restriction enzymes. Elsamicin A inhibits the rate of digestion by NruI (recognition sequence TCG/CGA) to a greater extent than it does for the other enzymes, thus evidencing the sequence-selective binding of elsamicin to CGC regions in DNA. Our results also show the important role of the neighbouring sequences in the elsamicin A-DNA interactions and their effects on the cleavage by restriction enzymes.
考虑到抗肿瘤抗生素埃尔斯米星A对AatII、ClaI、EcoRI、HindIII和NruI限制酶消化线性化pBR322 DNA速率的抑制作用，阐明了其与DNA结合的序列特异性。埃尔斯米星A对NruI（识别序列为TCG/CGA）消化速率的抑制作用比对其他酶的抑制作用更大，从而证明埃尔斯米星与DNA中CGC区域存在序列选择性结合。我们的结果还表明了相邻序列在埃尔斯米星A与DNA相互作用中的重要作用及其对限制酶切割的影响。
