Yamaguchi T, Franco-Saenz R, Mulrow P J
Department of Medicine, Medical College of Ohio, Toledo 43699.
Hypertension. 1992 Mar;19(3):263-9. doi: 10.1161/01.hyp.19.3.263.
Angiotensin II (Ang II) inhibits renin secretion and production from the kidney, but the effect of Ang II on adrenal renin is not clear. Nephrectomy, via elevated plasma adrenocorticotropic hormone (ACTH) and potassium, is a strong stimulator of adrenal renin production in the rat. This stimulation is inhibited by the infusion of Ang II, suggesting a negative feedback between Ang II and adrenal renin. In the present study, we examined the effect of Ang II on adrenal renin using a primary culture of rat glomerulosa cells. Cells were exposed to ACTH (10(-11) M), high potassium (8 and 12 mM), db-cyclic AMP (db-cAMP), (10(-3) M), or Ang II (10(-11) to 10(-5) M) for 24 hours, and active renin and inactive renin were measured. Active renin was predominant in the cells, whereas inactive renin predominated in the medium. Ang II stimulated renin production in a dose-dependent fashion (cell-active renin, 1.21 +/- 0.20 to 2.39 +/- 0.16; medium-inactive renin, 2.59 +/- 0.40 to 6.14 +/- 0.49 ng Ang I/10(6) cells). Both ACTH and db-cAMP significantly stimulated active renin in the cells (ACTH, 1.73 +/- 0.14 to 9.44 +/- 0.98; db-cAMP, 1.45 +/- 0.16 to 3.96 +/- 0.71 ng Ang I/10(6) cells) and inactive renin in the medium (ACTH, 4.98 +/- 0.38 to 43.7 +/- 5.63; db-cAMP, 3.80 +/- 0.32 to 33.55 +/- 5.62 ng Ang I/10(6) cells). The addition of Ang II (10(-7) M) blunted the stimulation of renin production by both ACTH and db-cAMP by 60%. High potassium-stimulated renin production was not inhibited by Ang II.(ABSTRACT TRUNCATED AT 250 WORDS)
血管紧张素II(Ang II)抑制肾脏中肾素的分泌和生成,但Ang II对肾上腺肾素的影响尚不清楚。通过升高血浆促肾上腺皮质激素(ACTH)和钾水平进行的肾切除术,是大鼠肾上腺肾素生成的强烈刺激因素。这种刺激会被Ang II输注所抑制,提示Ang II与肾上腺肾素之间存在负反馈。在本研究中,我们使用大鼠球状带细胞原代培养物研究了Ang II对肾上腺肾素的影响。将细胞暴露于ACTH(10⁻¹¹ M)、高钾(8和12 mM)、二丁酰环磷腺苷(db-cAMP)(10⁻³ M)或Ang II(10⁻¹¹至10⁻⁵ M)24小时,然后测量活性肾素和非活性肾素。细胞中以活性肾素为主,而培养基中以非活性肾素为主。Ang II以剂量依赖方式刺激肾素生成(细胞活性肾素,从1.21±0.20至2.39±0.16;培养基中非活性肾素,从2.59±0.40至6.14±0.49 ng Ang I/10⁶细胞)。ACTH和db-cAMP均显著刺激细胞中的活性肾素(ACTH,从1.73±0.14至9.44±0.98;db-cAMP,从1.45±0.16至3.96±0.71 ng Ang I/10⁶细胞)以及培养基中的非活性肾素(ACTH,从4.98±0.38至43.7±5.63;db-cAMP,从3.80±0.32至33.55±5.62 ng Ang I/10⁶细胞)。添加Ang II(10⁻⁷ M)使ACTH和db-cAMP对肾素生成的刺激作用减弱60%。高钾刺激的肾素生成未被Ang II抑制。(摘要截短于250词)