Gupta P, Franco-Saenz R, Mulrow P J
Department of Medicine, Medical College of Ohio, Toledo 43699-0008.
Endocrinology. 1992 Apr;130(4):2129-34. doi: 10.1210/endo.130.4.1312445.
The renin-angiotensin system consists of two main enzymes, renin and angiotensin-converting enzyme, which lead to the formation of angiotensin-II. Angiotensin-II is a potent vasoconstrictor and stimulates the production of aldosterone. In this study we examined the effect of ACTH, potassium, (Bu)2cAMP (dbcAMP), and catecholamines on the adrenal renin-angiotensin system. To study the production of renin and aldosterone in vitro, we developed a monolayer culture of bovine zona glomerulosa cells in serum-free medium. Collagenase-dispersed zona glomerulosa cells were incubated in Pasadena Foundation for Medical Research-4 medium containing 10% fetal calf serum for 72 h, and the medium was replaced with serum-free medium for the next 24 h of the experimental period. The cells during this 24 h were exposed to various doses of ACTH, potassium, dbcAMP, and sympathomimetic agents. ACTH and dbcAMP stimulated aldosterone secretion, and this secretion was associated with an increase in renin activity in cells and medium. Aldosterone was also stimulated by high doses of potassium, and potassium had a stimulatory effect on the secretion of renin in medium. Catecholamines had a weak stimulating effect on aldosterone secretion and were potent stimulators of adrenal renin activity in cells and medium. Dopamine had no significant effect on basal aldosterone secretion or renin activity in cells and medium. In conclusion, these data indicate that adrenal renin is synthesized in bovine zona glomerulosa cells in vitro, and that ACTH and dbcAMP stimulate adrenal renin and aldosterone production. Furthermore, adrenal renin, like renal renin, may be under the control of the sympathetic nervous system.
肾素-血管紧张素系统由两种主要酶，即肾素和血管紧张素转换酶组成，它们导致血管紧张素-II的形成。血管紧张素-II是一种强效血管收缩剂，并刺激醛固酮的产生。在本研究中，我们检测了促肾上腺皮质激素（ACTH）、钾、二丁酰环磷腺苷（dbcAMP）和儿茶酚胺对肾上腺肾素-血管紧张素系统的影响。为了在体外研究肾素和醛固酮的产生，我们在无血清培养基中建立了牛球状带细胞的单层培养。用胶原酶分散的球状带细胞在含有10%胎牛血清的帕萨迪纳医学研究基金会-4培养基中孵育72小时，然后在实验期的接下来24小时将培养基换成无血清培养基。在这24小时内，细胞暴露于不同剂量的ACTH、钾、dbcAMP和拟交感神经药。ACTH和dbcAMP刺激醛固酮分泌，这种分泌与细胞和培养基中肾素活性的增加有关。高剂量的钾也刺激醛固酮分泌，并且钾对培养基中肾素的分泌有刺激作用。儿茶酚胺对醛固酮分泌有微弱的刺激作用，并且是细胞和培养基中肾上腺肾素活性的强效刺激剂。多巴胺对细胞和培养基中的基础醛固酮分泌或肾素活性没有显著影响。总之，这些数据表明，肾上腺肾素在体外牛球状带细胞中合成，并且ACTH和dbcAMP刺激肾上腺肾素和醛固酮的产生。此外，肾上腺肾素，与肾肾素一样，可能受交感神经系统的控制。
