Cesselin F, Lagoguey M, Chauffournier J M, Guignard M, Desgrez P
Pathol Biol (Paris). 1976 Jan;24(1):41-6.
Five immunogenic substances were injected to rabbits. The method of immunisation by the intradermal route at 30 to 50 different points, with low doses of immunogen, without booster, does not seem to lead to production of antiserum of great specificity. The choice of the dose of immunogen to be injected is difficult, owing to our ignorance of the immunogenic dose for a given immunogen. The method of immunisation by the subcutaneous route with high doses followed by a booster, leads to more constant results, comparable to those obtained by the first method to which one may associate boosters by the subcutaneous route. The titers, affinities and specificities of the antisera thus produced are sufficient for the development of radio-immunoassay. The characteristics of the antisera obtained, related to the quality of the immunogen, do not seem to depend on the method of immunisation.
将五种免疫原性物质注射到兔子体内。采用皮内途径在30至50个不同部位注射低剂量免疫原且不进行加强免疫的免疫方法，似乎不会产生具有高度特异性的抗血清。由于我们对特定免疫原的免疫原剂量一无所知，所以很难选择要注射的免疫原剂量。皮下途径高剂量注射并随后进行加强免疫的免疫方法会产生更稳定的结果，这与通过皮下途径进行加强免疫的第一种方法所获得的结果相当。如此产生的抗血清的效价、亲和力和特异性足以用于放射免疫分析的开展。所获得的抗血清的特性与免疫原的质量有关，似乎并不取决于免疫方法。
