Tanaka Norie, Nishikawa Kazutaka, Ishimaru Kanji
Venture Business Laboratory and Department of Applied Biological Sciences, Faculty of Agriculture, Saga University, 1 Honjo, Saga 840-8502, Japan.
J Agric Food Chem. 2003 Sep 24;51(20):5906-10. doi: 10.1021/jf030267s.
Radical scavenging activities of extracts and constituents in Cornus capitata adventitious root cultures were evaluated by using 1,1-diphenyl-2-pycrylhydrazyl (DPPH) and superoxide anion radicals. Inhibitory activity against peroxidation of linoleic acid was assayed by using the thiobarbituric acid (TBA) method. Ethyl acetate and aqueous fractions were prepared from adventitious roots cultured in Murashige-Skoog liquid medium with 0.1 microM Cu(2+) (0.1CuMS) or 10 microM Cu(2+) (10CuMS). The highest scavenging activities on DPPH and superoxide anion radicals were observed in the ethyl acetate fraction from 0.1CuMS. In the inhibitory activity against linoleic acid oxidation, the ethyl acetate fraction from 10CuMS was highest among the fractions tested. The ethyl acetate fraction of adventitious roots cultured in 0.1CuMS contained mainly galloylglucoses (1,2,3,6-tetragalloylglucose and 1,2,3,4,6-pentagalloylglucose). The ethyl acetate fraction of adventitious roots cultured in 10CuMS contained mainly ellagic acid derivatives [3,3'-di-O-methylellagic acid 4-(5"-acetyl)-alpha-L-arabinofuranoside and stenophyllin H1]. Aqueous fractions prepared from both media contained iridoid glycosides (dihydrocornin and cornin). Tetra- and pentagalloylglucoses showed strong inhibitory activities (61.9 and 85.2%, respectively) against linoleic acid oxidation relative to those of butylated hydroxytoluene (BHT) (91.1%) or alpha-tocopherol (49.5%) at 50 microM concentration. Although both ellagic acid derivatives had weak activities (<50%) on DPPH and superoxide anion radical scavenging, 3,3'-di-O-methylellagic acid 4-(5"-acetyl)-alpha-L-arabinofuranoside was stronger (74.7%) than alpha-tocopherol (49.5%) in inhibiting linoleic acid oxidation at 50 microM concentration. Iridoid glycosides exhibited little activity against DPPH and superoxide anion radicals or against oxidation of linoleic acid.
采用1,1-二苯基-2-苦基肼（DPPH）和超氧阴离子自由基，对头状四照花不定根培养物中的提取物和成分的自由基清除活性进行了评估。采用硫代巴比妥酸（TBA）法测定了对亚油酸过氧化的抑制活性。从在含有0.1微摩尔铜离子（0.1CuMS）或10微摩尔铜离子（10CuMS）的Murashige-Skoog液体培养基中培养的不定根中制备乙酸乙酯和水相部分。在来自0.1CuMS的乙酸乙酯部分中观察到对DPPH和超氧阴离子自由基的最高清除活性。在对亚油酸氧化的抑制活性方面，来自10CuMS的乙酸乙酯部分在测试的部分中最高。在0.1CuMS中培养的不定根的乙酸乙酯部分主要含有没食子酰葡萄糖（1,2,3,6-四没食子酰葡萄糖和1,2,3,4,6-五没食子酰葡萄糖）。在10CuMS中培养的不定根的乙酸乙酯部分主要含有鞣花酸衍生物[3,3'-二-O-甲基鞣花酸4-(5"-乙酰基)-α-L-阿拉伯呋喃糖苷和狭叶番荔枝素H1]。从两种培养基中制备的水相部分都含有环烯醚萜苷（二氢山茱萸素和山茱萸素）。在50微摩尔浓度下，四没食子酰葡萄糖和五没食子酰葡萄糖对亚油酸氧化表现出较强的抑制活性（分别为61.9%和85.2%），相对于丁基化羟基甲苯（BHT）（91.1%）或α-生育酚（49.5%）。尽管两种鞣花酸衍生物对DPPH和超氧阴离子自由基清除活性较弱（<50%），但在浓度为50微摩尔时，3,3'-二-O-甲基鞣花酸4-(5"-乙酰基)-α-L-阿拉伯呋喃糖苷在抑制亚油酸氧化方面比α-生育酚（49.5%）更强（74.7%）。环烯醚萜苷对DPPH和超氧阴离子自由基或亚油酸氧化几乎没有活性。
