Dhalwal Kamlesh, Deshpande Yogesh S, Purohit Ajit P
Department of Pharmacognosy, Poona College of Pharmacy, Bharati Vidyapeeth University, Erandwane, Pune, India.
J Med Food. 2007 Dec;10(4):683-8. doi: 10.1089/jmf.2006.129.
Sida rhombifolia (L.) ssp. retusa (L.) is widely used in Ayurvedic medicine for the treatment of fever as well as a diuretic. The comparative antioxidant potentials of ethanol extract of roots, stems, leaves, and whole plant were studied. Estimation of total polyphenolic content and high-performance thin-layer chromatography profile were determined. Further inhibition of oxygen-derived free radicals, viz., assays for free radical scavenging, reducing power, superoxide anion scavenging, nitric oxide scavenging, and anti-lipid peroxidation, were performed. All the antioxidant activities were compared with standard antioxidants such as butylated hydroxyanisole and alpha-tocopherol acetate. Extracts were found to be good scavengers of the 1,1-diphenyl-2-picrylhydrazyl radical in the order root > leaves > whole plant > stem with 50% inhibitory concentrations of 546.1, 852.8, 983.8, and 1,222.5 microg/mL, respectively. All extracts of this plant showed effective free radical scavenging activity, reducing power, and superoxide scavenging activity. Only root extract inhibited lipid peroxidation in rat liver and brain homogenate. All these antioxidant properties were concentration dependent. In addition, total polyphenolic contents of all the extracts were determined as gallic acid equivalents. The highest antioxidant activity was observed in root extract. The results obtained from the current study indicate that S. rhombifolia ssp. retusa is a potential source of natural antioxidants.
菱叶马松子（Sida rhombifolia (L.) ssp. retusa (L.)）在阿育吠陀医学中被广泛用于治疗发热以及作为利尿剂。研究了该植物根、茎、叶和全株乙醇提取物的比较抗氧化潜力。测定了总多酚含量和高效薄层色谱图谱。进一步进行了对氧衍生自由基的抑制作用，即自由基清除、还原能力、超氧阴离子清除、一氧化氮清除和抗脂质过氧化测定。所有抗氧化活性均与丁基羟基茴香醚和醋酸生育酚等标准抗氧化剂进行了比较。发现提取物是1,1-二苯基-2-苦基肼自由基的良好清除剂，清除顺序为根＞叶＞全株＞茎，50%抑制浓度分别为546.1、852.8、983.8和1222.5微克/毫升。该植物的所有提取物均表现出有效的自由基清除活性、还原能力和超氧阴离子清除活性。只有根提取物能抑制大鼠肝脏和脑匀浆中的脂质过氧化。所有这些抗氧化特性均呈浓度依赖性。此外，所有提取物的总多酚含量均以没食子酸当量测定。根提取物中观察到最高的抗氧化活性。本研究所得结果表明，菱叶马松子是天然抗氧化剂的潜在来源。
