Gancel Anne-Laure, Ollitrault Patrick, Froelicher Yann, Tomi Felix, Jacquemond Camille, Luro Francois, Brillouet Jean-Marc
Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Département FLHOR, TA50/16, F-34398 Montpellier Cedex 5, France.
J Agric Food Chem. 2003 Sep 24;51(20):6006-13. doi: 10.1021/jf0345090.
Volatile compounds were extracted by a pentane/ether (1:1) mixture from the leaves of seven citrus somatic tetraploid hybrids sharing mandarin as their common parent and having lime, Eurêka lemon, lac lemon, sweet orange, grapefruit, kumquat, or poncirus as the other parent. Extracts were examined by GC-MS and compared with those of their respective parents. All hybrids were like their mandarin parent, and unlike their nonmandarin parents, in being unable to synthesize monoterpene aldehydes and alcohols. The hybrids did retain the ability, although strongly reduced, of their nonmandarin parents to synthesize sesquiterpene hydrocarbons, alcohols, and aldehydes. These results suggest that complex forms of dominance in the mandarin genome determine the biosynthesis pathways of volatile compounds in tetraploid hybrids. A down-regulation of the biosynthesis of methyl N-methylanthranilate, a mandarin-specific compound, originates from the genomes of the nonmandarin parents. Statistical analyses showed that all of the hybrids were similar to their common mandarin parent in the relative composition of their volatile compounds.
用戊烷/乙醚（1:1）混合物从七个柑橘体细胞四倍体杂种的叶片中提取挥发性化合物，这些杂种都以宽皮橘为共同亲本，另一个亲本分别为来檬、尤力克柠檬、印度酸橙、甜橙、葡萄柚、金橘或枳。提取物通过气相色谱-质谱联用仪进行检测，并与其各自亲本的提取物进行比较。所有杂种都与它们的宽皮橘亲本相似，与非宽皮橘亲本不同，无法合成单萜醛和醇。杂种确实保留了其非宽皮橘亲本合成倍半萜烃、醇和醛的能力，尽管这种能力大幅降低。这些结果表明，宽皮橘基因组中复杂的显性形式决定了四倍体杂种中挥发性化合物的生物合成途径。一种宽皮橘特有的化合物——N-甲基邻氨基苯甲酸甲酯生物合成的下调源自非宽皮橘亲本的基因组。统计分析表明，所有杂种在挥发性化合物的相对组成上都与其共同的宽皮橘亲本相似。
