Delarue Jacques, Labarthe Francois, Cohen Richard
Laboratoire Régional de Nutrition Humaine & EA-948, Médecine 4-Nutrition, CHU Cavale Blanche, F-29200-Brest, France.
Br J Nutr. 2003 Oct;90(4):777-86. doi: 10.1079/bjn2003964.
The present study examined the effects of a 3-week fish-oil supplementation (6 g/d) on the rate of plasma glucose disappearance (Rd glucose), hepatic glucose production (HGP), carbohydrate oxidation and lipid oxidation during exercise. Six untrained males (23+/-1 years; 67.6+/-2.7 kg) performed two 90 min cycling exercise sessions at 60 % of maximal O2 output separated by 20 d. During the 20 d before the first test, they ingested 6 g olive oil/d, then 6 g fish oil/d during the 20 d before the second test. Plasma glucose fluxes and lipolysis were traced using 6,6-[(2)H2]glucose and 1,1,2,3,3-[(2)H5]glycerol respectively. Substrates oxidation was obtained from indirect calorimetry. At rest HGP and the Rd glucose were similar after olive oil and fish oil (1.83 (SE 0.05) v. 1.67 (SE 0.11) mg/kg per min). During exercise, fish oil reduced the stimulation of both the Rd glucose (5.06 (SE 0.23) v. 6.37 (SE 0.12) mg/kg per min; P<0.05) and HGP (4.88 (SE 0.24) v. 5.91 (SE 0.21) mg/kg per min; P<0.05). Fish oil also reduced glucose metabolic clearance rate (6.93 (SE 0.29) v. 8.30 (SE 0.57) ml/min). Carbohydrate oxidation tended to be less stimulated by exercise after fish oil than after olive oil (12.09 (SE 0.60) v. 13.86 (se 1.11) mg/kg per min; NS). Lipid oxidation tended to be more stimulated by exercise after fish oil (7.34 (SE 0.45) v. 6.85 (SE 0.17) mg/kg per min; NS). Glycaemia, lactataemia, insulinaemia and glucagonaemia were similarly affected by exercise after fish oil and olive oil. Lipolysis at rest was similar after fish oil and olive oil (2.92 (SE 0.42) v. 2.94 (SE 0.28) micromol/kg per min) and similarly stimulated by exercise (6.42 (SE 0.75) v. 6.77 (SE 0.72) micromol/kg per min). It is concluded that fish oil reduced the Rd glucose by 26 % by reducing glucose metabolic clearance rate, possibly by facilitating fat oxidation, and reduced HGP by 21%, possibly by a feedback mechanism.
本研究检测了为期3周补充鱼油（6克/天）对运动期间血浆葡萄糖消失率（Rd葡萄糖）、肝脏葡萄糖生成（HGP）、碳水化合物氧化和脂质氧化的影响。6名未经训练的男性（23±1岁；67.6±2.7千克）进行了两次90分钟的自行车运动，运动强度为最大摄氧量的60%，两次运动间隔20天。在第一次测试前的20天里，他们每天摄入6克橄榄油，然后在第二次测试前的20天里，每天摄入6克鱼油。分别使用6,6-[(2)H2]葡萄糖和1,1,2,3,3-[(2)H5]甘油追踪血浆葡萄糖通量和脂肪分解。底物氧化通过间接量热法获得。静息时，摄入橄榄油和鱼油后HGP和Rd葡萄糖相似（1.83（标准误0.05）对1.67（标准误0.11）毫克/千克每分钟）。运动期间，鱼油降低了对Rd葡萄糖（5.06（标准误0.23）对6.37（标准误0.12）毫克/千克每分钟；P<0.05）和HGP（4.88（标准误0.24）对5.91（标准误0.21）毫克/千克每分钟；P<0.05）的刺激。鱼油还降低了葡萄糖代谢清除率（6.93（标准误0.29）对8.30（标准误0.57）毫升/分钟）。与摄入橄榄油后相比，摄入鱼油后运动对碳水化合物氧化的刺激作用趋于减弱（12.09（标准误0.60）对13.86（标准误1.11）毫克/千克每分钟；无显著性差异）。与摄入橄榄油后相比，摄入鱼油后运动对脂质氧化的刺激作用趋于增强（7.34（标准误0.45）对6.85（标准误0.17）毫克/千克每分钟；无显著性差异）。摄入鱼油和橄榄油后，运动对血糖、血乳酸、胰岛素血症和胰高血糖素血症的影响相似。静息时脂肪分解在摄入鱼油和橄榄油后相似（2.92（标准误0.42）对2.94（标准误0.28）微摩尔/千克每分钟），运动时受到的刺激也相似（分别为6.42（标准误0.75）和6.77（标准误0.72）微摩尔/千克每分钟）。研究得出结论，鱼油通过降低葡萄糖代谢清除率使Rd葡萄糖降低26%，可能是通过促进脂肪氧化实现的，并且使HGP降低21%，可能是通过反馈机制实现的。
