Park Shi-Nae, Yeo Sang W, Park Kyung-Ho, Chung Min-Kyo, Lee Heung-Youp, Chae Sayong
Otolaryngology-HNS, Kangnam St. Mary's Hospital, The Catholic University of Korea, College of Medicine, Seoul, Korea.
Am J Otolaryngol. 2003 Sep-Oct;24(5):323-7. doi: 10.1016/s0196-0709(03)00063-2.
The objective of this study was to investigate a protective system of pediatric palatine tonsils and adenoids against superoxide radicals (O(2)(-)) and to evaluate the clinical factors including otitis media with effusion that are related to both the expression and activities of copper zinc superoxide dismutases (CuZnSODs).
CuZnSODs in the palatine tonsils and adenoids were studied using immunohistochemistry and reverse-transcription polymerase chain reaction.
Immunohistochemistry showed that CuZnSODs distribute in the crypt epithelium, mucous membrane, mantle zone, and extrafollicular area of the pediatric adenoids as well as in the palatine tonsils. Otitis media with effusion and paranasal sinusitis were related to CuZnSODs expression in the pediatric adenoids. In addition, the frequency of tonsillitis and presence of recent tonsillitis were significantly related to CuZnSODs expression in the pediatric tonsils (P <.05).
In both the pediatric palatine tonsils and adenoids, the mechanism of tissue protection against infection and frequent inflammatory reactions may be closely related to CuZnSODs expression. There may be close relationship between the increased level of O(2)(-) that leads to an increase in CuZnSODs in the pediatric adenoid tissue and the development of otitis media.
本研究的目的是探究小儿腭扁桃体和腺样体针对超氧阴离子自由基（O₂⁻）的保护系统，并评估包括分泌性中耳炎在内的与铜锌超氧化物歧化酶（CuZnSODs）表达及活性相关的临床因素。
采用免疫组织化学和逆转录聚合酶链反应研究腭扁桃体和腺样体中的CuZnSODs。
免疫组织化学显示，CuZnSODs分布于小儿腺样体的隐窝上皮、黏膜、套区和滤泡外区域以及腭扁桃体中。分泌性中耳炎和鼻窦炎与小儿腺样体中CuZnSODs的表达有关。此外，扁桃体炎的发生频率和近期是否患扁桃体炎与小儿扁桃体中CuZnSODs的表达显著相关（P<.05）。
在小儿腭扁桃体和腺样体中，组织抵御感染和频繁炎症反应的保护机制可能与CuZnSODs的表达密切相关。小儿腺样体组织中导致CuZnSODs增加的O₂⁻水平升高与中耳炎的发生之间可能存在密切关系。
