Miller M A, Bokhari S A, Qadir K, Raza A
Department of Internal Medicine, University of Cincinnati Medical Center, Ohio.
J Histochem Cytochem. 1992 Mar;40(3):427-30. doi: 10.1177/40.3.1313063.
We describe an immunohistochemical technique that makes use of two monoclonal antibodies (MAb), one to detect the transforming growth factor B (TGFB) and another that reacts with iodo- and bromodeoxyuridine. The purpose of this technique is to determine the relationship between TGFB expression and the S-phase cells in human tumors. Since both can be distinctly identified in situ from tissue embedded in plastic, in assessment of the geographic orientation of S-phase cells in relation to such factors as TGFB, contiguity to blood vessels, nerve fibers, and macrophages can also be achieved.
我们描述了一种免疫组织化学技术，该技术利用两种单克隆抗体（MAb），一种用于检测转化生长因子β（TGFB），另一种与碘脱氧尿苷和溴脱氧尿苷发生反应。这项技术的目的是确定人类肿瘤中TGFB表达与S期细胞之间的关系。由于两者都可以在塑料包埋的组织中原位清晰识别，因此在评估S期细胞相对于TGFB等因素的空间定位时，还可以实现与血管、神经纤维和巨噬细胞的邻近性评估。
